COORDINATE CONTROL OF HUMAN IA ANTIGENS AND II CHAIN

作为T细胞受体的主要配体之一，II类主要的 组织相容性抗原是唯一适合于控制这两种细胞的抗原。 T细胞的分化和活化。 Ia表达差异 可以赋予独特的能力，删除自身反应性T细胞在 胸腺，或在外周启动淋巴因子级联反应。 这两 对于肿瘤、感染因子和可溶性 抗原 因此我们调节免疫反应的能力 多种抗原最终依赖于我们对II类基因的理解 调控 本文的目的是研究协调调节 多个第二类基因之间，并与不变链 基因 累积的证据表明，II类基因和不变量 基因有一些同源元件。 相反，一些II类基因也 具有独特的调控因素。 所有的调节元件都可以 与假定为转录因子核蛋白相互作用。 本提案的目的是确定DNA结合蛋白是否 调节一个II类基因（DR α）的基因也调节各种其他基因， II类基因以及不变链基因。 为了实现这一点， 也将使用相关的DNA结合蛋白。 每个DNA的能力 对DR α基因具有特异性的结合蛋白， II类基因以及不变链基因以协调的方式 将被评估。 这种类型的功能分析肯定会 确定II类基因是否由于共享的 对DNA结合蛋白的特异性。

As one of the primary ligands for the T cell receptor, the Class II major histocompatibility antigens are uniquely suited to control both the differentiation and activation of T cells. Differences in Ia expression can confer unique capabilities to delete self-reactive T cells in the thymus, or to initiate lymphokine cascades in the periphery. Both of these important for immune surveillance of tumors, infectious agents and soluble antigens. Therefore our ability to modulate the immune response to a variety of antigens ultimately relies on our understanding of class II gene regulation. The goal of this proposal is study the coordinate regulation of multiple class II genes among themselves and with the invariant chain gene. Accumulative evidence suggests that class II genes and invariant gene share some homologous elements. Conversely, some class II genes also have unique regulatory elements. All of the regulatory elements can interact with nuclear proteins which are presume transcription factors. The objectives of this proposal are to determine if DNA-binding proteins that regulate one class II gene (DRalpha) also regulates various other class II genes, as well as the invariant chain gene. To achieve this, relevant DNA binding proteins will also be used. The ability of each DNA binding protein with specificity for the DRalpha gene to control multiple class II genes as well as the invariant chain gene in a coordinate fashion will be assessed. This type of functional analysis will definitely determine if class II genes are coordinately regulated due to shared specificity for DNA-binding proteins.