CONSEQUENCES OF SIV INFECTION OF MACROPHAGES

巨噬细胞 SIV 感染的后果

• 批准号: 3144793
• 负责人: DOUGLAS J RINGLER
• 金额: $ 15.08万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-03-01 至 1995-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3144793
• 关键词: HIV infections; Macaca; alveolar macrophages; cellular immunity; cytokine; immunoregulation; leukocyte activation /transformation; mixed lymphocyte reaction test; molecular genetics; phagocytosis; simian AIDSs; simian immunodeficiency virus; virus cytopathogenic effect; virus replication

DESCRIPTION: (Adapted from the applicant's abstract) The objectives of this proposal are to examine the effect of viral infection on macrophages in greater detail, using the alveolar macrophage and SIV as a basis of study. Specifically, the aims of this proposal are to: 1) to study the microenvironmental influences of cytokines and activation agents on the efficiency of viral replication within SIV-infected alveolar macrophages, 2) to study the function of SIV-infected alveolar macrophages, as determined by their ability to present antigen and participate in autologous mixed lymphocyte reactions, ability to phagocytize and kill bacteria, ability to perform cytotoxic functions against neoplastic and parasitic targets, and the ability to produce cytokines and immunoregulatory molecules upon stimulation, 3) study any alterations in macrophage-related gene expression resulting from SIV infection and investigate the molecular basis for these alterations, 4) study the in vivo significance of macrophage tropism by comparing the pathogenic potential of SIV isolates that propagate well within macrophages in vitro with isolates that do not grow at all within this particular cell, and 5) investigate the molecular determinants responsible for the growth of SIV within macrophages.

描述：(改编自申请人的摘要)目标 这项建议是为了检查病毒感染对巨噬细胞的影响 更详细地说，使用肺泡巨噬细胞和SIV作为基础 学习。具体来说，这项建议的目的是：1)研究 细胞因子和激活剂对细胞微环境的影响 病毒在SIV感染的肺泡巨噬细胞内复制的效率， 2)研究SIV感染的肺泡巨噬细胞的功能。 由它们呈递抗原和参与 自体混合淋巴细胞反应、吞噬和杀伤能力 细菌，对肿瘤和肿瘤执行细胞毒性功能的能力 寄生靶标，以及产生细胞因子和 免疫调节分子在刺激下，3)研究任何变化 SIV感染引起的巨噬细胞相关基因表达和 研究这些改变的分子基础，4)体内研究 比较巨噬细胞致病潜能对巨噬细胞趋向性的意义 用分离株在巨噬细胞内繁殖良好的SIV分离株 在这个特定的细胞内根本不生长，并调查 SIV在体内生长的分子决定因素 巨噬细胞。