Continued Support of an spf Macaca nemestrina breeding colony

摘要 本申请的目的是寻求桥梁资金，以维持健康，福祉，并继续 免费延长期间约翰·霍普金斯猪尾猕猴种群的SPF状态，直至 获得成功的续期申请。在此期间，JHU殖民地将继续 提供健康，行为正常，MHC特征，SPF猪尾猕猴为NIH资助 艾滋病毒/艾滋病相关研究。我们将继续我们的计划，扩大目前的SPF繁殖区， 增加用于繁殖的雌性数量和可向NIH销售的幼年雄性和雌性数量- 资助了约翰霍普金斯大学和其他研究机构的艾滋病毒/艾滋病研究人员。 我们将继续进行基本的病原体检测，包括定期进行结核病皮肤测试和病毒检测 筛选以确保殖民地对以下病毒因子保持SPF：猕猴疱疹病毒1型 （B病毒）、猿猴免疫缺陷病毒（SIV）、猿猴逆转录病毒（SRV）和猿猴T淋巴细胞营养性病毒（T-Lymphotrophic virus）。 病毒（STLV），以及筛选SARS-CoV-2感染的殖民地。我们已经进行了一项 根据审查者对我们以前的报告的反馈， 更新申请，以及扩大设施的功能能力，以继续殖民 增长除了笼子更换项目外，该项目的资金来自 以前未使用的U42资金，并将在无费用延长期之前完成，这些 改进由JHU内部资助，并将在此过渡期间继续快速进行。

Abstract The aim of this application is to seek bridge funding to maintain the health, well-being, and continued SPF status of the Johns Hopkins pig-tailed macaque colony during the period of no-cost extension, until the award of a successful renewal application. During this period, the JHU colony will continue to provide healthy, behaviorally normal, MHC-characterized, SPF pig-tailed macaques for NIH-funded HIV/AIDS related research. We will continue our plan of expanding the current SPF breeding colony to increase the numbers of females for breeding and juvenile males and females available for sale to NIH- funded HIV/AIDS investigators both within Johns Hopkins University and at other research institutions. We will continue to conduct basic pathogen testing, to include regular tuberculosis skin tests and viral screening to ensure the colony remains SPF for the following viral agents: Cercopithecine herpesvirus 1 (B virus), Simian Immunodeficiency Virus (SIV), Simian Retrovirus (SRV), and Simian T-lymphotrophic Virus (STLV), as well as screening the colony for SARS-CoV-2 infection. We have already undertaken a number of facility and programmatic improvements in response to reviewer feedback on our previous renewal application, as well as expanding the functional capacity of the facility for continued colony growth. With the exception of the caging replacement project, which was funded by the use of previously unspent U42 funds and will be completed prior to the no-cost extension period, these improvements are funded internally by JHU and will continue apace during this bridge period.