SALIVARY SECRETION IN A BLOOD-SUCKING ARTHROPOD

吸血节肢动物的唾液分泌

• 批准号: 3139840
• 负责人: JOHN ROBERT SAUER
• 金额: $ 11.41万
• 依托单位: OKLAHOMA STATE UNIVERSITY STILLWATER
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 1993-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3139840
• 关键词: arthropod borne communicable disease; arthropod nonpollutant control; biochemistry; calcium; cell membrane; cyclic AMP; diacylglycerols; disease vectors; dopamine receptor; eating; electron microscopy; enzyme linked immunosorbent assay; enzyme mechanism; fluorescence spectrometry; gel electrophoresis; gel filtration chromatography; high performance liquid chromatography; hydrolysis; ion exchange chromatography; laboratory mouse; laboratory rabbit; lipid metabolism; membrane lipids; phorbols; phosphatidylinositols; phosphomonoesterases; phosphoprotein phosphatase; physiology; prostaglandins; protein kinase C; receptor sensitivity; salivary glands; salivation; scintillation counter; sheep; ticks

Studies of the physiology and biochemistry and control of salivary secretion in feeding ixodid ticks will be continued. After reception of the primary stimulus (dopamine), the salivary glands utilize cyclic AMP (cAMP) and Ca2+ to help modulate cell activity during secretion. Little is known about how Ca2+ is mobilized during salivary gland function. Therefore, a role for the metabolism of plasma membrane phosphoinositides in signal transduction in the salivary glands will be studied. The question of whether enhanced hydrolysis of plasma membrane phosphatidylinositol 4,5-trisphosphate after agonist-receptor interaction mediates mobilization of intracellular Ca2+ will be explored. The physiological agonists of salivary gland phosphoinositide metabolism will be identified. The ability of these agonists to modulate salivary secretion with and without other known agonists of secretion i.e. dopamine and cAMP will be studied. The possible relationship between agonist-stimulated turnover of phosphoinositides and production and secretion of prostaglandins will be investigated. Possible protein kinase C activity in the salivary glands and its activation by another product of phosphoinositide metabolism, diacylglycerol and phorbol esters will be investigated. The "second messenger" role for cAMP has been studied but little is known about functions of phosphoproteins. Therefore, the phosphoprotein phosphorylated by cAMP-dependent protein kinase to modulate protein phosphatase activity and its relationship to inhibition of protein phosphatase by Ca2+ will be investigated. Information about how metabolic pathways regulate secretion, either directly or indirectly, should shed light on synthesis and secretion of substances that contribute to a tick's successful feeding and their ability to vector pathogens. Ultimately the results may be useful for applied biology. Interference with an essential point in a metabolic pathway controlling salivary secretion could provide a new approach to tick control or the pathogens they vector.

唾液分泌的生理生化及控制研究 进食硬蜱中的分泌将继续。 在接受主要刺激（多巴胺）后，唾液 腺体利用环磷酸腺苷（cAMP）和Ca 2+来帮助调节细胞 分泌期间的活动。 关于Ca 2+是如何在 在唾液腺功能期间动员。 因此，对于 信号转导中质膜磷酸肌醇代谢 将研究唾液腺中的转导。 问题 是否增强了质膜的水解 激动剂受体后4，5-三磷酸磷脂酰肌醇 相互作用介导的细胞内Ca 2+的动员将是 探讨了 唾液腺的生理激动剂 将鉴定磷酸肌醇代谢。 的能力 这些激动剂调节唾液分泌， 其它已知的分泌激动剂，即多巴胺和cAMP， 研究了 激动剂刺激与 磷酸肌醇的周转以及 将对兰丁进行调查。 可能是蛋白激酶C 唾液腺中的活性及其被另一种 磷酸肌醇代谢产物，二酰基甘油和 将研究佛波醇酯。 cAMP的“第二信使”作用已被研究，但很少 关于磷蛋白的功能是已知的。 因此 cAMP依赖性蛋白激酶磷酸化磷蛋白 调节蛋白磷酸酶活性及其与 将研究Ca 2+对蛋白磷酸酶的抑制作用。 关于代谢途径如何调节分泌的信息， 直接或间接地，应该阐明综合和 分泌的物质，有助于蜱的成功 进食和传播病原体的能力。 最终 结果可能对应用生物学有用。 干扰 控制唾液分泌的代谢途径的关键点 分泌物可以提供一种新的方法来控制蜱虫， 它们携带的病原体