RELATION OF STRUCTURE TO FUNCTION OF ENZYMES

酶的结构与功能的关系

• 批准号: 3150737
• 负责人: EDMOND H FISCHER
• 金额: $ 17.81万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-09-01 至 1988-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3150737
• 关键词: X ray crystallography; affinity chromatography; biochemical evolution; brain; carbohydrate metabolism; chemical structure function; cyclic AMP; electron microscopy; enzyme induction /repression; enzyme structure; gel electrophoresis; hormone regulation /control mechanism; human tissue; immunochemistry; insulin; mass spectrometry; molecular pathology; monoclonal antibody; muscle; muscle contraction; muscle proteins; muscular dystrophy; phosphatase inhibitor; phosphorylase phosphatase; phosphorylation; protein kinase; radioimmunoassay; radiotracer; scintillation counter

We propose to continue our studies on the structure and function of enzymes involved in the regulating of carbohydrate metabolism in relation to hormone action and muscle contraction. Specifically, we are interested in: 1) The architecture of phosphorylase phosphatase, an enzyme that might play a central role in the control of glycogen synthesis and breakdown and the possible involvement of insulin in its regulation. The enzyme exists in an inactive form and the various mechanisms by which it can be activated are being investigated. 2) Crystallizing the cAMP-dependent protein kinase catalytic subunit and determining the structure and function of phosphorylase kinase with particular emphasis on the characterization of its catalytic sites and the role of its other subunits. 3) The structure and physiological function of a heat-stable protein inhibitor of protein kinase that has been isolated from skeletal muscle. A new project concerned with microtubule cold-stability has been initiated. Attempts are being made to isolate and characterize a number of microtubule-associated proteins involved in this process and to determine how their activity might be modulated by phosphorylation-dephosphorylation reactions catalyzed by calmodulin-dependent and independent kinases.

我们建议继续对酶的结构和功能进行研究 与调节碳水化合物代谢有关 荷尔蒙作用和肌肉收缩。具体地说，我们有兴趣 In：1)磷酸化酶磷酸酶的结构，一种可能 在控制糖原合成和分解中发挥核心作用 胰岛素可能参与其调节。这种酶存在 处于非活动状态以及可通过其激活的各种机制 正在接受调查。2)晶化cAMP依赖的蛋白激酶 催化亚基及其结构和功能的测定 磷酸化酶激酶，特别强调其特性 它的催化部位和它的其他亚基的作用。3)结构 一种耐热蛋白抑制物的生理功能 从骨骼肌中分离出来的一种激酶。一个新项目 关于微管的冷稳定性研究已经开始。尝试次数为 被用来分离和鉴定许多微管相关的 参与这一过程的蛋白质，并确定它们的活性如何 受以下酶催化的磷酸化-去磷酸化反应的调节 钙调蛋白依赖和独立的激酶。

项目成果

Binding of 5,5'-bis[8-(phenylamino)-1-naphthalenesulfonate] by the regulatory subunits of adenosine cyclic 3',5'-phosphate dependent protein kinase.

腺苷环状 3,5-磷酸依赖性蛋白激酶的调节亚基与 5,5-双[8-(苯氨基)-1-萘磺酸] 的结合。

• DOI: 10.1021/bi00265a029
• 发表时间: 1982
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Bohnert,JL;Malencik,DA;Anderson,SR;Teller,D;Fischer,EH
• 通讯作者: Fischer,EH

Calcium-regulatory mechanisms. Functional classification using skinned fibers.

钙调节机制。

• DOI: 10.1085/jgp.77.2.177
• 发表时间: 1981
• 期刊: The Journal of general physiology
• 作者: Kerrick,WG;Hoar,PE;Cassidy,PS;Bolles,L;Malencik,DA
• 通讯作者: Malencik,DA

Purification and characterization of sheep brain cold-stable microtubules.

羊脑冷稳定微管的纯化和表征。

• DOI: 10.1073/pnas.80.6.1560
• 发表时间: 1983
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Pirollet,F;Job,D;Fischer,EH;Margolis,RL
• 通讯作者: Margolis,RL

Purification of the major protein-tyrosine-phosphatases of human placenta.

• DOI: 10.1016/s0021-9258(18)68702-2
• 发表时间: 1988-05
• 期刊: The Journal of biological chemistry
• 作者: N. Tonks;C. D. Diltz;E. Fischer

Reconstitution of types I and II adenosine cyclic 3',5'-phosphate dependent protein kinase.

• DOI: 10.1021/bi00265a028
• 发表时间: 1982-10
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: J. L. Bohnert;D. Malencik;S. Anderson;D. Teller;E. Fischer