PARATHYROID HORMONE CELLULAR ACTION, CAMP, AND BIOASSAY

该项目的目的是研究甲状旁腺的作用机制。 激素(PTH)主要集中在cAMP的作用，以及 基于骨组织腺苷环化酶刺激的生物测定。 建立基于骨组织刺激的甲状旁腺素生物测定方法 腺苷环化酶，我们建议表征腺苷环化酶。 高反应性骨肉瘤克隆(ROS 17/2)：最佳检测方法 最大化激素反应的条件(镁ATP2-、镁离子、GTP)和比较 不同物种的甲状旁腺素以及合成的甲状旁腺素片段和 类比。 我们建议研究以前报道的腺苷酸环化酶的作用。 提高细胞溶质因子对激素的作用(Egan等，BBRC 80：176-182,1978) 使用：(1)反应性和非反应性克隆和 (2)胞浆因子和激素反应在体内的平行诱导性。 实验工具。

The objective of the project is to study the mechanisms of action of parathyroid hormone (PTH) focusing primarily on the role of cAMP, and the development of a bioassay based on stimulation of adenylate cyclase from osseous tissue. For establishing the PTH bioassay based on stimulation of osseous tissue adenylate cyclase, we propose to characterize the adenylate cyclase of the highly responsive osteosarcoma clone (ROS 17/2) with respect to: optimum assay conditions for maximizing hormone response (MgATP2-, Mg2ion, GTP) and compare effects of PTH from various species as well as synthetic PTH fragments and analogs. We propose to investigate the role of the previously dscovered adenylate cyclase enhancing cytosol factor (Egan et al., BBRC 80:176-182, 1978) on hormone stimulation of the enzyme using: (1) responsive and non responsive clones and (2) the parallel in vivo inducibility of cytosol factor and hormone response, as experimental tools.