SERINE AND THREONINE METABOLISM

这项研究的长期目标是描述新陈代谢 哺乳动物肝组织中丝氨酸、苏氨酸和甘氨酸的调节 在患病和正常状态下。我们将特别强调 黄曲霉毒素分解代谢中催化特定步骤的酶的关系 这些氨基酸以及这些酶与新生的关系 不稳定的甲基。大豆对丝氨酸、苏氨酸和甘氨酸的代谢 哺乳动物的肝脏组织将从它们的角度进行研究 1-碳代谢中的连锁作用和可能的作用。其中许多 酶有磷酸吡哆醛和/或叶酸作为辅助因子。我们会 研究这些酶的催化作用机理。光谱研究和 动力学研究将使我们能够进一步表征这些反应。 关于这些物质的分布和划分的证据 还将对酶进行研究。

The long term goal of this research is to characterize the metabolic regulation of serine, threonine and glycine in mammalian liver tissue and in diseased and normal states. Special emphasis will be placed on the relationship of the enzymes catalyzing committed steps in the catabolism of these amino acids and on the relationship of these enzymes to neogenesis of labile methyl groups. The metabolism of serine, threonine and glycine in mammalian liver tissue will be studied from the standpoint of their interlocking and putative roles on 1-carbon metabolism. Many of these enzymes have as co-factors pyridoxal phosphate and/or folic acid. We will study the mechanism of catalysis by these enzymes. Spectral studies and kinetic studies will enable us to further characterize these reactions. Evidence concerning the distribution and compartmentalization of these enzymes will also be investigated.