SERINE AND THREONINE METABOLISM

丝氨酸和苏氨酸代谢

• 批准号: 3151039
• 负责人: LEODIS DAVIS
• 金额: $ 9.42万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-03-01 至 1986-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3151039
• 关键词: 5,10 methylenetetrahydrofolate; aminoacid metabolism; atherosclerosis; chemical chain length; chromatography; enzyme substrate; glutamates; glycine; isozymes; liver metabolism; methyl group; mitochondria; purine /pyrimidine metabolism; radiotracer; serine; threonine

The long term goal of this research is to characterize the metabolic regulation of serine, threonine and glycine in mammalian liver tissue and in diseased and normal states. Special emphasis will be placed on the relationship of the enzymes catalyzing committed steps in the catabolism of these amino acids and on the relationship of these enzymes to neogenesis of labile methyl groups. The metabolism of serine, threonine and glycine in mammalian liver tissue will be studied from the standpoint of their interlocking and putative roles on 1-carbon metabolism. Many of these enzymes have as co-factors pyridoxal phosphate and/or folic acid. We will study the mechanism of catalysis by these enzymes. Spectral studies and kinetic studies will enable us to further characterize these reactions. Evidence concerning the distribution and compartmentalization of these enzymes will also be investigated.

这项研究的长期目标是表征代谢 哺乳动物肝组织中丝氨酸、苏氨酸和甘氨酸的调节， 在患病和正常状态下。 将特别强调 酶催化的关键步骤之间的关系 这些氨基酸和这些酶的关系，以新生的 不稳定的甲基。 丝氨酸、苏氨酸和甘氨酸的代谢 哺乳动物的肝脏组织将从他们的立场进行研究， 在1-碳代谢中的连锁和推定作用。 许多这些 酶具有磷酸吡哆醛和/或叶酸作为辅因子。 我们将 研究这些酶的催化机理。 光谱研究和 动力学研究将使我们能够进一步表征这些反应。 关于这些物质的分布和划分的证据 还将研究酶。

项目成果

Purification and properties of aminoacetone synthetase from beef liver mitochondria.

牛肝线粒体氨基丙酮合成酶的纯化及其性质。

• 发表时间: 1986
• 期刊: The Journal of biological chemistry
• 作者: Fubara,B;Eckenrode,F;Tressel,T;Davis,L
• 通讯作者: Davis,L

Decarboxylation of glycine by serine hydroxymethyltransferase in the presence of lipoic acid.

在硫辛酸存在下，丝氨酸羟甲基转移酶使甘氨酸脱羧。

• 发表时间: 1983
• 期刊: The Journal of biological chemistry
• 作者: Zieske,LR;Davis,L
• 通讯作者: Davis,L

Interaction between L-threonine dehydrogenase and aminoacetone synthetase and mechanism of aminoacetone production.

L-苏氨酸脱氢酶和氨基丙酮合成酶之间的相互作用以及氨基丙酮产生的机制。

• 发表时间: 1986
• 期刊: The Journal of biological chemistry
• 作者: Tressel,T;Thompson,R;Zieske,LR;Menendez,MI;Davis,L
• 通讯作者: Davis,L