GLYCINE, SERINE AND THREONINE METABOLISM

甘氨酸、丝氨酸和苏氨酸代谢

• 批准号: 3225681
• 负责人: LEODIS DAVIS
• 金额: $ 15.42万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-03-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3225681
• 关键词: acetone; acyltransferase; aminoacid metabolism; chromatography; cow; enzyme complex; enzyme mechanism; enzyme structure; enzyme substrate; folate; glycine; isozymes; liver metabolism; methyl group; mitochondria; pyridoxal phosphate; serine; thin layer chromatography; threonine

The goal of this research is to characterize the metabolic regulation of serine, threonine and glycine in mammalian liver tissue and in diseased and normal states. Special emphasis will be placed on the relationship of the enzymes catalyzing committed steps in the catabolism of these amino acids and on the relationship of these enzymes to neogenesis of labile methyl groups. The metabolism of serine, threonine and glycine in mammalian liver tissue will be studied from the standpoint of their interlocking and putative roles on 1-carbon metabolism. Many of these enzymes have as co-factors pyridoxal phosphate and/or folic acid. We will study the mechanism of catalysis by these enzymes. Spectral studies and kinetic studies will enable us to further characterize these reactions. Evidence concerning the distribution and compartmentalization of these enzymes will also be investigated. The role of aminoacetone in metabolism will be investigated. Special attention will be directed towards unraveling the metabolic rate of the carbon atoms of the amino acids; glycine, serine and threonine. The enzymes identified for investigation include, glycine cleavage complex, serine transhydroxymethylase, aminoacetone synthase, and thronine dehydrogenase. A multi-faceted approach will be taken which will include: organic synthesis, enzyme isolation, kinetic measurements and mechanistic studies.

本研究的目的是描述代谢调节的特点， 丝氨酸、苏氨酸和甘氨酸在哺乳动物肝组织和患病和 正常状态。 将特别强调的关系， 催化这些氨基酸分解的关键步骤的酶 以及这些酶与不稳定甲基的新生的关系 组 哺乳动物肝脏中丝氨酸、苏氨酸和甘氨酸的代谢 组织将从它们的互锁的观点来研究， 对1-碳代谢的假定作用。 这些酶中的许多具有 辅因子磷酸吡哆醛和/或叶酸。 我们会研究 这些酶的催化机制。 光谱研究和动力学 研究将使我们能够进一步描述这些反应。 证据 关于这些酶的分布和区室化， 也被调查。 氨基丙酮在代谢中的作用将是 研究了 将特别注意解开 氨基酸的碳原子的代谢率;甘氨酸，丝氨酸和 苏氨酸。 确定用于研究的酶包括甘氨酸 切割复合物，丝氨酸转羟甲基化酶，氨基丙酮合酶，和 硫氨酸脱氢酶。 将采取多方面的办法， 包括：有机合成、酶分离、动力学测量， 机械研究。

项目成果

Purification and structural characterization of porcine L-threonine dehydrogenase.

猪L-苏氨酸脱氢酶的纯化和结构表征。

• DOI: 10.1006/prep.1994.1061
• 发表时间: 1994
• 期刊: Protein expression and purification
• 影响因子: 1.6
• 作者: Kao,YC;Davis,L
• 通讯作者: Davis,L

2-Amino-3-ketobutyrate-CoA ligase from beef liver mitochondria. Purification and partial sequence.

来自牛肝线粒体的 2-氨基-3-酮丁酸-CoA 连接酶。

• 发表时间: 1994
• 期刊: The Journal of biological chemistry
• 作者: Tong,H;Davis,L
• 通讯作者: Davis,L

2-Amino-3-ketobutyrate-CoA ligase from beef liver mitochondria: an NMR spectroscopic study of low-barrier hydrogen bonds of a pyridoxal 5'-phosphate-dependent enzyme.

来自牛肝线粒体的 2-氨基-3-酮丁酸-CoA 连接酶：吡哆醛 5-磷酸依赖性酶的低势垒氢键的 NMR 光谱研究。

• DOI: 10.1021/bi00010a027
• 发表时间: 1995
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Tong,H;Davis,L
• 通讯作者: Davis,L

Cofactor identification of threonine-serine dehydratase from sheep liver.

绵羊肝脏苏氨酸-丝氨酸脱水酶的辅因子鉴定。

• DOI: 10.1006/prep.1993.1058
• 发表时间: 1993
• 期刊: Protein expression and purification
• 影响因子: 1.6
• 作者: Tong,H;Davis,L
• 通讯作者: Davis,L