ROLE OF THYROTROPIN IN THYROID CANCER

我们将继续致力于阐明 TSH-受体的相互作用和生物信号的传播 病理性甲状腺。 将特别强调阐明鸟苷的作用机理。 核苷酸调节TSH受体复合体与腺苷的偶联 循环酶。我们计划将注意力集中在腺苷环化酶的解剖上 系统通过溶解和提纯三个主要成分中的每一个 系统：TSH受体、鸟苷核苷酸结合蛋白和催化单元。通过 可溶组分的重组，我们希望建立 络合物和与之相关的分子事件的化学计量学 激素受体占据TSH受体，因此，与 受体-效应器耦合。

Our efforts will continue to be directed toward elucidating the mechanisms of TSH-receptor interaction and propagation of the biological signal in normal and pathologic thyroid glands. Special emphasis will be placed on elucidating the mechanism by which guanyl nucleotides modulate the coupling of TSH-receptor complexes to adenylate cyclase. We plan to focus our attention on dissecting the adenylate cyclase system by solubilizing and purifying each of the three main components of the system: TSH receptor, guanyl nucleotide binding protein and catalytic unit. By reconstitution of the solubilized components, we hope to establish the stoichiometry of the complex and the molecular events associated with the hormone receptor occupancy of the TSH receptors and, hence, with receptor-effector coupling.