BONE CELL CALCIUM REGULATION

骨细胞中钙调节的性质将用 特别强调钙、环核苷酸的相互关系 和钙调蛋白。丰富的破骨细胞和成骨细胞种群 细胞将通过胎儿和胎儿的胶原酶顺序消化获得 对新生大鼠颅骨进行流式细胞术检测。环状AMP和 GMP采用放射免疫法测定。钙的运输将会是 用45Ca在完整细胞和质膜囊泡中测量。这个 骨吸收药和骨吸收抑制剂对细胞周期的影响 将研究特定细胞群体中的GMP水平以及 环状GMP对钙转运的影响。骨骼中钙调素活性的研究 细胞将通过放射免疫分析进行评估，并对 抗钙调素药物对cAMP、cGMP调节和钙的影响 将对运输进行研究。钙调素在调节中的作用 将直接研究腺苷/鸟苷环化酶和钙转运 在浓缩膜组分中。从这些研究中获得的信息 应进一步阐明钙和环核苷酸在 控制骨代谢。这些研究也应该提供一个更清晰的 对控制骨骼的生化过程的理解 新陈代谢，并可能在理论上导致更合理的方法来 代谢性骨病的治疗。

The nature of calcium regulation in bone cells will be studied with specific emphasis on the interrelationships of calcium, cyclic nucleotides and calmodulin. Enriched populations of osteoclastic and osteoblastic cells will be obtained by sequential collagenase digestions of fetal and newborn rat calvaria followed by flow cytometry studies. Cyclic AMP and GMP will be measured by radioimmunoassay. Calcium transport will be measured in intact cells and plasma membrane vesicles with 45Ca. The effects of bone resorptive agents and inhibitors of resorption on cyclic GMP levels in characterized cell populations will be studied as well as the effects of cyclic GMP on calcium transport. Calmodulin activity in bone cells will be assessed by radioimmunoassay and the effects of anti-calmodulin drugs on cyclic AMP, cyclic GMP regulation and calcium transport will be studied. The role of calmodulin in regulating adenylate/guanylate cyclase and calcium transport will be studied directly in enriched membrane fractions. The information obtained in these studies should further elucidate the role of calcium and cyclic nucleotides in the control of bone metabolism. The studies should also provide a clearer understanding of the biochemical processes involved in control of bone metabolism and could theoretically lead to a more rationale approach to the treatment of metabolic bone disease.