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HORMONAL REGULATION OF ISOLATED GASTRIC MUCOSAL CELLS

离体胃粘膜细胞的激素调节

基本信息

批准号：
3152081
负责人：
GARY C ROSENFELD
金额：
$ 9.59万
依托单位：
UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
依托单位国家：
美国
项目类别：
财政年份：
1983
资助国家：
美国
起止时间：
1983-05-01 至 1986-04-30
项目状态：
已结题

项目摘要

The objective of this research is to understand how secretagogues and secretory inhibitors regulate and coordinate the secretion and production of hydrogen ions from parietal cells isolated from the rat gastric mucosa. Enriched and purified parietal cells will be used to study secretagogue stimulation of acid secretion as well as related biochemical and cytoskeletal modifications associated with receptor activation. We plan to detect and more precisely characterize several cell membrane receptors using techniques of radiolabeled ligand binding. We also plan to examine biochemically the role of cyclic nucleotide metabolism, calmodulin, and cytoskeletal proteins in cell regulation by secretagogues. We will test our hypothesis that hydrogen ion production stimulated by acetylcholine and histamine is due to an interaction between calcium regulated intracellular events and accumulated cyclic AMP. Additionally, immunofluorescent cytochemical techniques will be used to localize components of both the cyclic nucleotide metabolic system and cytoskeletal structure which may be specifically involved in parietal cell function. This research should lead to a better understanding of the regulation of gastric acid secretion. Also, this research should identify alternative loci for possible pharmacological intervention necessary to control hydrogen ion secretion and peptic disease.

本研究的目的是了解促分泌素和分泌抑制剂调节和协调分泌和生产从大鼠胃粘膜分离的壁细胞的氢离子。富集和纯化的壁细胞将用于研究促分泌素刺激酸分泌以及相关生化和与受体活化相关的细胞骨架修饰。我们计划检测并更精确地表征几种细胞膜受体使用放射性标记的配体结合技术。我们还计划检查生物化学的作用，环核苷酸代谢，钙调素，细胞骨架蛋白通过促分泌素调节细胞。我们将测试我们的假设是，乙酰胆碱刺激氢离子产生，组胺是由于细胞内钙调节事件和累积的环AMP。此外，免疫荧光细胞化学技术将用于定位这两种成分，环核苷酸代谢系统和细胞骨架结构，特别是参与壁细胞的功能。这项研究将导致以更好地了解胃酸分泌的调节。此外，这项研究应该确定可能的替代位点，控制氢离子分泌所需的药物干预和消化性疾病。