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IN VITRO METHODS FOR ISLET ISOLATION AND TRANSPLANTATION

胰岛分离和移植的体外方法

基本信息

批准号：
3152461
负责人：
ORION D HEGRE
金额：
$ 8.83万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
1983
资助国家：
美国
起止时间：
1983-04-01 至 1986-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3152461
关键词：
chromatography collagenase embryo /fetus growth media homologous transplantation immunochemistry oxidation pancreatic islet transplantation polyamines radioimmunoassay radiotracer scintillation spectrometry serum tissue /cell culture tissue /cell preparation tissue /organ preservation xenotransplantation

项目摘要

These studies represent a continuation of our work in the field of experimental diabetes, pancreatic and islet culture, and islet tissue transplantation. They are designed to investigate the utility of a new technique for the isolation and purification of islet tissue from perinatal sources, as it relates to studies of islet physiology and non-syngeneic transplantability. Advantages inherent in this new technique will be assessed against techniques currently available, and in addition the role of certain cytotoxic byproducts of metabolism as a consequence of standard tissue culture methodology will be examined for their possible role in he alteration of islet tissue immunogenicity. Taking advantage of the selective toxicity of various cell types to products of polyamine oxidation, attempts will be made to design culture conditions which produce purified endocrine tissue. This tissue will be tested in non-syngeneic transplantation models to assess its capacity to permanently reverse experimentally induced diabetes in non-immunosuppressed recipients. Using techniques of immunoassay, ultrastructural analysis, immunocytochemistry, quantitative morphometry, spectrophotometry, chromatography, enzymology and tissue culture, morphological as well as biochemical and physiological parameters can be correlated. These attempts at deriving solutions to the problems of islet supply and non-syngeneic transplantability in experimental animals may hasten the clinical application of islet transplantation to man.

这些研究代表了我们在以下领域工作的继续：实验性糖尿病，胰腺和胰岛培养，以及胰岛组织移植他们的目的是调查一种新的围产期胰岛组织的分离纯化技术来源，因为它涉及到胰岛生理学和非同源的研究可移植性这种新技术的固有优点是根据目前可用的技术进行评估，此外，某些细胞毒性代谢副产物作为标准的结果组织培养方法学将被检查它们在他的可能的作用胰岛组织免疫原性改变。利用了不同类型细胞对多胺产物的选择性毒性氧化，尝试设计培养条件，纯化的内分泌组织该组织将在非同源移植模型，以评估其永久逆转在非免疫抑制受者中实验诱导的糖尿病。使用免疫分析、超微结构分析、免疫细胞化学、定量形态测定法、分光光度法、色谱法、酶学和组织培养、形态学以及生化和生理参数可以相互关联。这些试图解决胰岛供应和非同基因移植的问题，实验动物可加速胰岛的临床应用移植到人身上。