权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

NITROXIDE FREE RADICAL CONTRAST MEDIA FOR NMR IMAGING

用于 NMR 成像的氮氧自由基对比介质

基本信息

批准号：
3152384
负责人：
Robert Charles Brasch
金额：
$ 19.8万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
1983
资助国家：
美国
起止时间：
1983-02-01 至 1986-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3152384
关键词：
contrast media nitrous oxide radiodiagnosis toxicology

项目摘要

Nuclear magnetic resonance (NMR) imaging is an emerging diagnostic tool capable of tomographic imaging of humans without the use of ionizing radiation. Two NMR imagers, developed in our department, utilize the magnetic behavior of hydrogen nuclei and depict their distribution and magnetic relaxation characteristic within bodily tissues. The aims of this project are to better define by animal experimentation the diagnostic benefits, the toxicity, and the pharamacology of nitroxide stable free radicals (NSFR) used in vivo as NMR contrast agents. The proposed investigations require the combined disciplines of diagnostic imaging, synthetic chemistry, physics and pharmacology. Preliminary studies in animals show that NSFR, a group of organic paramagnetic compounds used as contrast media can enhance the NMR contrast differences between urinary structures and surrounding tissues and permit evaluation of renal function. Tested NSFR are rapidly excreted by glomerular filtration and appear within urinary structures to produce a dramatic increase in the NMR intensity signal. NSFR, like many radiographic contrast media, are largely excluded from the central nervous system by the normally functioning blood-brain barrier. It is postulated that these contrast media will penetrate into the brain in pathologic conditions and thereby aid in the NMR identification of neurologic diseases. To achieve other organ-specific targetting, NSFR may be covalently conjugated to biomolecules including proteins and lipids. Aggregated particles of NSFR-protein and/or NSFR-liposomes may be diagnostically useful in NMR imaging of the liver and spleen by depicting the functional reticulo- endothelial system. These contrast-enhancing applications of NSFR would substantially add to the diagnostic utility of NMR imaging. Preliminary toxicity studies indicate safety of NSFR. Toxic doses far exceed those necessary for contrast enhancement. The long-term goal is to develop clinically useful NMR contrast agents for use in the diagnosis of human diseases.

核磁共振成像是一种新兴的诊断工具能够在不使用电离的情况下对人类进行断层成像，辐射两个核磁共振成像仪，在我们的部门开发，利用氢核的磁性行为，并描绘它们的分布，身体组织内的磁弛豫特性。其目的是项目是通过动物实验更好地定义诊断利益，毒性，和药理学的氮氧化物稳定的自由自由基（NSFR）在体内用作NMR造影剂。拟议调查需要诊断成像的组合学科，合成化学、物理学和药理学。初步研究动物表明，NSFR，一组有机顺磁性化合物，对比剂可以增强尿液与尿液之间的核磁共振对比度差异结构和周围组织，并允许评估肾脏功能测试的NSFR通过肾小球过滤迅速排出，出现在泌尿结构中，以产生NMR的急剧增加，强度信号。 NSFR与许多放射造影剂一样，从中枢神经系统中排除，血脑屏障假设这些造影剂将在病理条件下渗透到大脑中，从而有助于神经系统疾病的核磁共振鉴别。实现其他器官特异性为了靶向，NSFR可以共价缀合至生物分子，包括蛋白质和脂质。 NSFR-蛋白和/或 NSFR-脂质体可用于肝脏的NMR成像诊断，脾脏描绘功能性网状内皮系统。这些 NSFR的对比度增强应用将大大增加核磁共振成像的诊断效用。初步毒性研究表明 NSFR的安全性毒性剂量远远超过造影剂所需的剂量增强长期目标是开发临床上有用的核磁共振用于诊断人类疾病的造影剂。