权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

CYTOSOL ACTIVATOR OF RAT RETICULOCYTE ADENYLATE CYCLASE

大鼠网状细胞腺苷酸环化酶的胞质激活剂

基本信息

批准号：
3152193
负责人：
Elizabeth J Shane
金额：
$ 6.49万
依托单位：
COLUMBIA UNIV NEW YORK MORNINGSIDE
依托单位国家：
美国
项目类别：
财政年份：
1981
资助国家：
美国
起止时间：
1981-09-01 至 1989-08-31
项目状态：
已结题

项目摘要

The objective of this research project is to establish the identity, mechanism of action, and importance of the cellular regulator of adenylate cyclase obtained from the cytosol of rat reticulocytes. The reticulocyte cytosol activator protein (RCAP), that augments catecholamine-sensitive adenylate cyclase in rat reticulocyte membranes, has been only partially purified and characterized. The goals of the research plan are to purify and to completely characterize RCAP; to elucidate the mechanisms by which it so profoundly influences catecholamine responsiveness; and to establish to what extent, and by what means, RCAP regulates adenylate cyclase activity in other tissues and species. RCAP will be purified to homogeneity using established biochemical techniques including preparative isoelectric focusing and other specific approaches outlined in Methods. The mechanism of action of RCAP will be investigated by studies directed at RCAP's effects upon each of the three components of the hormonal unit, the beta-adrenergic receptor, the guanine nucleotide coupling protein, and the catalytic unit of adenylate cyclase, as well as the interactions among them. Specific methods will include radioligand binding studies, determination of adenylate cyclase activity, and assessment of the guanine nucleotide binding protein by means of the ADP-ribosylation assay. The effects of RCAP upon other hormones that stimulate or inhibit adenylate cyclase and in several other mammalian and tissues will also be investigated in order to determine the importance of RCAP as a general regulator of hormone action.

本研究项目的目标是建立身份，作用机制和腺苷酸细胞调节剂的重要性从大鼠网织红细胞的胞浆中获得的环化酶。网织红细胞胞质溶胶激活蛋白（RCAP），增强儿茶酚胺敏感性腺苷酸环化酶在大鼠网织红细胞膜，一直只是部分纯化和表征。研究计划的目标是并完全表征RCAP;阐明其机制，它如此深刻地影响了儿茶酚胺的反应;并建立在何种程度上，通过何种方式，RCAP调节腺苷酸环化酶在其他组织和物种中的活性。 RCAP将被纯化，使用已建立的生物化学技术，包括制备等电聚焦和方法中概述的其他特定方法。 RCAP的作用机制将通过以下研究进行研究： RCAP对激素单位的三个组成部分中的每一个的影响， β-肾上腺素能受体，鸟嘌呤核苷酸偶联蛋白，和腺苷酸环化酶的催化单位，以及他们具体方法包括放射性配体结合研究，腺苷酸环化酶活性测定和鸟嘌呤通过ADP-核糖基化测定的核苷酸结合蛋白。的 RCAP对其他刺激或抑制腺苷酸的激素的影响环化酶和其他几种哺乳动物和组织中也将被调查，以确定RCAP作为一般的重要性，激素作用的调节器。