ACID-BASE REGULATION OF RENAL METABOLISM
肾脏代谢的酸碱调节
基本信息
- 批准号:3152642
- 负责人:
- 金额:$ 6.79万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1983
- 资助国家:美国
- 起止时间:1983-08-01 至 1986-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The increased synthesis of ammonia and glucose by the kidney in acidosis is
regulated by both short-term and long-term controls. In the short-term it
is known that increased flux through the pathway in vitro can be brought
about by certain hormones, a decrease in the medium pH or even the addition
of serum from an acidotic animal. The long-term response to acidosis
involves induction of certain key enzymes, phosphoenolpyruvate
carboxykinase, glutaminase and glutamate dehydrogenase. The induction of
phosphoenolpyruvate carboxykinase is known to be due to increased rates of
enzyme synthesis and this correlates with increased amounts of mRNA coding
for the enzyme. The mechanism by which acid-base balance can cause such
changes is unknown. The aim of the work described in this application is
to determine how acidosis can influence the levels of phosphoenolpyruvate
carboxykinase in the kidney. The work will use both rats and chickens
where differences in intracellular enzyme distribution and different
regulatory patterns will allow comparison of mechanisms. The initial work
will characterize the in vivo response to acidosis. Determination of
extracellular signals possibly involved in the acidosis response will be
carried out using isolated proximal tubule cells where changes in the
concentrations of mRNA coding for phosphoenolpyruvate carboxykinase are
readily detectable. Having identified extracellular agents the work will
progress to understanding their intracellular mechanism of action. This
will include experiments to determine if these agents act by changing
transcription rates for the enzyme. The long-term goal is to determine if
a common mechanism is involved in the acid-base mediated changes in the
levels of a number of renal enzymes. In particular the identification of
the "signal" to the kidney during acidosis is of fundamental importance to
the understanding of the regulation of renal metabolism.
酸中毒时肾脏对氨和葡萄糖的合成增加
既有短期控制,也有长期控制。在短期内,
已知通过体外途径的增加的通量可以带来
大约是由于某些激素,介质pH的降低,甚至是添加
一种酸性动物的血清。对酸中毒的长期反应
涉及某些关键酶的诱导,磷酸烯醇式丙酮酸
羧酸激酶、谷氨酰胺酶和谷氨酸脱氢酶。的归纳
已知的是,磷酸烯醇式丙酮酸羧酸激酶是由于
酶的合成,这与mRNA编码量的增加有关
为了这种酶。酸碱平衡导致这种情况的机制
变化是未知的。本申请中描述的工作的目的是
确定酸中毒如何影响磷烯醇式丙酮酸水平
肾脏中的羧酸激酶。这项工作将同时使用老鼠和鸡
其中细胞内酶分布的差异和不同
监管模式将允许对机制进行比较。最初的工作
将表征体内对酸中毒的反应。的决定
可能参与酸中毒反应的细胞外信号将是
使用分离的近端小管细胞进行实验,其中
编码磷酸烯醇式丙酮酸羧激酶的mRNA浓度为
容易察觉的。在确定了胞外因子之后,这项工作将
了解其细胞内作用机制的进展。这
将包括实验,以确定这些代理是否通过改变
酶的转录速率。长期目标是确定是否
一种共同的机制涉及到酸-碱介导的细胞周期变化。
一些肾脏酶的水平。尤其是识别出
酸中毒时对肾脏的“信号”对
对肾脏代谢调节的认识。
项目成果
期刊论文数量(7)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
The regulation of glutamine and ketone-body metabolism in the small intestine of the long-term (40-day) streptozotocin-diabetic rat.
长期(40天)链脲佐菌素糖尿病大鼠小肠谷氨酰胺和酮体代谢的调节。
- DOI:10.1042/bj2420061
- 发表时间:1987
- 期刊:
- 影响因子:0
- 作者:Watford,M;Erbelding,EJ;Smith,EM
- 通讯作者:Smith,EM
Hormonal and acid-base regulation of phosphoenolpyruvate carboxykinase mRNA levels in rat kidney.
大鼠肾脏中磷酸烯醇丙酮酸羧激酶 mRNA 水平的激素和酸碱调节。
- DOI:10.1016/0003-9861(90)90135-l
- 发表时间:1990
- 期刊:
- 影响因子:3.9
- 作者:Watford,M;Mapes,RE
- 通讯作者:Mapes,RE
The synthesis of glucose and ammonia by kidney tubules isolated from suckling and early-weaned lambs.
从哺乳羔羊和早期断奶羔羊身上分离出的肾小管合成葡萄糖和氨。
- DOI:10.1016/0305-0491(87)90212-4
- 发表时间:1987
- 期刊:
- 影响因子:0
- 作者:Watford,M;Lane,SF;Hogue,DE
- 通讯作者:Hogue,DE
Effects of metabolic acidosis and diabetes on the abundance of specific renal mRNAs.
代谢性酸中毒和糖尿病对特定肾 mRNA 丰度的影响。
- DOI:10.1016/0020-711x(89)90187-0
- 发表时间:1989
- 期刊:
- 影响因子:0
- 作者:Mapes,RE;Watford,M
- 通讯作者:Watford,M
Hormonal and nutritional regulation of phosphoenolpyruvate carboxykinase mRNA levels in chicken kidney.
鸡肾磷酸烯醇丙酮酸羧激酶 mRNA 水平的激素和营养调节。
- DOI:10.1093/jn/119.2.319
- 发表时间:1989
- 期刊:
- 影响因子:0
- 作者:Watford,M
- 通讯作者:Watford,M
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
MALCOLM WATFORD其他文献
MALCOLM WATFORD的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('MALCOLM WATFORD', 18)}}的其他基金
相似国自然基金
肿瘤微环境因子Lactic acidosis在肿瘤细胞耐受葡萄糖剥夺中的作用机制研究
- 批准号:81301707
- 批准年份:2013
- 资助金额:23.0 万元
- 项目类别:青年科学基金项目
相似海外基金
Identification of factor to induce lactic acidosis in pre-metastatic niche
转移前微环境中诱导乳酸性酸中毒的因素的鉴定
- 批准号:
23K06620 - 财政年份:2023
- 资助金额:
$ 6.79万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Carbonic Anhydrase IX Acts as a Novel CO2/HCO3- Sensor and Protects the Pulmonary Endothelial Barrier from Acidosis
碳酸酐酶 IX 作为新型 CO2/HCO3- 传感器并保护肺内皮屏障免受酸中毒的影响
- 批准号:
10678442 - 财政年份:2023
- 资助金额:
$ 6.79万 - 项目类别:
Investigation based on both basic and clinical study about acidosis caused by piganide, SGLT2 inhibitor and surgical stress
皮甘尼、SGLT2抑制剂和手术应激引起的酸中毒的基础和临床研究
- 批准号:
23K08372 - 财政年份:2023
- 资助金额:
$ 6.79万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Role of proton-sensing G-protein-coupled receptors in the regulation of microglia and microvessel endothelial cell function in brain acidosis in a mouse ischemia reperfusion model.
质子感应 G 蛋白偶联受体在小鼠缺血再灌注模型脑酸中毒中调节小胶质细胞和微血管内皮细胞功能的作用。
- 批准号:
22K07342 - 财政年份:2022
- 资助金额:
$ 6.79万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Magnetic Resonance Fingerprinting of Tumor Vascular Perfusion and Acidosis
肿瘤血管灌注和酸中毒的磁共振指纹图谱
- 批准号:
10593285 - 财政年份:2022
- 资助金额:
$ 6.79万 - 项目类别:
Acidosis in pulmonary endothelial injury and repair
酸中毒与肺内皮损伤与修复
- 批准号:
10341493 - 财政年份:2022
- 资助金额:
$ 6.79万 - 项目类别:
Acidosis in pulmonary endothelial injury and repair
酸中毒与肺内皮损伤与修复
- 批准号:
10558528 - 财政年份:2022
- 资助金额:
$ 6.79万 - 项目类别:
Characterization of an abundant lactate-utilizing Campylobacter involved in mitigating rumen acidosis
参与减轻瘤胃酸中毒的丰富乳酸利用弯曲杆菌的表征
- 批准号:
557929-2021 - 财政年份:2022
- 资助金额:
$ 6.79万 - 项目类别:
Postgraduate Scholarships - Doctoral
Impact of metabolic acidosis on muscle mitochondrial energetics, metabolic health and physical endurance in persons with chronic kidney disease
代谢性酸中毒对慢性肾病患者肌肉线粒体能量学、代谢健康和身体耐力的影响
- 批准号:
10278747 - 财政年份:2021
- 资助金额:
$ 6.79万 - 项目类别:
Impact of metabolic acidosis on muscle mitochondrial energetics, metabolic health and physical endurance in persons with chronic kidney disease
代谢性酸中毒对慢性肾病患者肌肉线粒体能量学、代谢健康和身体耐力的影响
- 批准号:
10671682 - 财政年份:2021
- 资助金额:
$ 6.79万 - 项目类别:














{{item.name}}会员




