CHARACTERIZATION OF SUB-EPITHELIAL MATRIX ANTIGENS

上皮下基质抗原的表征

• 批准号: 3154136
• 负责人: ROBERT E BURGESON
• 金额: $ 5.68万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-23 至 1988-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3154136
• 关键词: antibody; basement membrane; electron microscopy; enzyme linked immunosorbent assay; epidermolysis bullosa; epithelium; extracellular matrix; fibroblasts; gel electrophoresis; human tissue; immunofluorescence technique; monoclonal antibody; skin; skin disorder; surface antigens; tissue /cell culture

The basement membrane zone underlying the squamous epithelium of external tissues is ultrastructurally more complex than basement membrane zones of other tissues. In addition to lamina densa, these regions contain additional cellular elements such as the hemidesmosomes of the basal cells, anchoring filaments between the hemidesmosomes and the lamina densa, anchoring fibrils which appear to connect the lamina densa with the underlying connective tissue, and oxytalan fibers which may mediate the interaction of elastic elements with the lamina densa. The diseases of bullous pemphigoid and epidermolysis bullosa affect specific components in this region, and demonstrate the uniqueness of these structures to external tissues. As a first attempt to describe the network of molecules involved in the interaction of epithelial cells with molecules deep in the underlying matrix, we have produced monoclonal antibodies to yet unrecognized components. Two of these antibodies have been selected for further characterization. One of them have immunological and biochemical characteristics similar to those expected for anchoring filaments. The other has characteristics resembling oxytalan fibers. We have shown that these antigens are synthesized by specific cell populations and have demonstrated the feasibility of their chemical characterization. We propose to isolate and characterize these antigens in detail, to identify the antigen with ultrastructurally observed fibrous components of the basement membrane zone, and to investigate potential interactions between these components and other known connective tissue elements. We anticipate that the proposed studies will provide specific information regarding these basement membrane zone proteins and will help form a basis for the understanding of the structure of this unique region. This information will provide the background for forming hypotheses regarding the molecular events underlying the pathogenesis of epidermolysis bullosa and bullous pemphigoid.

外表皮鳞状上皮下的基底膜区

项目成果

Type XII and XIV collagens mediate interactions between banded collagen fibers in vitro and may modulate extracellular matrix deformability.

• DOI: 10.1016/s0021-9258(18)46913-x
• 发表时间: 1994-11
• 期刊: The Journal of biological chemistry
• 作者: Toshio Nishiyama;A. M. McDonough;R. Bruns;R. Burgeson

The collagens of skin.

皮肤的胶原蛋白。

• 发表时间: 1987
• 期刊: Current problems in dermatology
• 作者: Burgeson,RE

Large complex globular domains of type VII procollagen contribute to the structure of anchoring fibrils.

VII 型原胶原的大型复杂球状结构域有助于锚定原纤维的结构。

• 发表时间: 1986
• 期刊: The Journal of biological chemistry
• 作者: Lunstrum,GP;Sakai,LY;Keene,DR;Morris,NP;Burgeson,RE
• 通讯作者: Burgeson,RE

Type III collagen can be present on banded collagen fibrils regardless of fibril diameter.

• DOI: 10.1083/jcb.105.5.2393
• 发表时间: 1987-11
• 期刊: The Journal of cell biology
• 作者: Keene DR;Sakai LY;Bächinger HP;Burgeson RE
• 通讯作者: Burgeson RE

Anchoring fibrils contain the carboxyl-terminal globular domain of type VII procollagen, but lack the amino-terminal globular domain.

锚定原纤维含有 VII 型原胶原的羧基端球状结构域，但缺乏氨基端球状结构域。

• 发表时间: 1987
• 期刊: The Journal of biological chemistry
• 作者: Lunstrum,GP;Kuo,HJ;Rosenbaum,LM;Keene,DR;Glanville,RW;Sakai,LY;Burgeson,RE
• 通讯作者: Burgeson,RE