CHARACTERIZATION OF SUB-EPITHELIAL MATRIX ANTIGENS

上皮下基质抗原的表征

• 批准号: 3157342
• 负责人: ROBERT E BURGESON
• 金额: $ 5.71万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-23 至 1988-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3157342
• 关键词: antibody; basement membrane; electron microscopy; enzyme linked immunosorbent assay; epidermolysis bullosa; epithelium; extracellular matrix; fibroblasts; gel electrophoresis; human tissue; immunofluorescence technique; monoclonal antibody; skin; skin disorder; surface antigens; tissue /cell culture

The basement membrane zone underlying the squamous epithelium of external tissues is ultrastructurally more complex than basement membrane zones of other tissues. In addition to lamina densa, these regions contain additional cellular elements such as the hemidesmosomes of the basal cells, anchoring filaments between the hemidesmosomes and the lamina densa, anchoring fibrils which appear to connect the lamina densa with the underlying connective tissue, and oxytalan fibers which may mediate the interaction of elastic elements with the lamina densa. The diseases of bullous pemphigoid and epidermolysis bullosa affect specific components in this region, and demonstrate the uniqueness of these structures to external tissues. As a first attempt to describe the network of molecules involved in the interaction of epithelial cells with molecules deep in the underlying matrix, we have produced monoclonal antibodies to yet unrecognized components. Two of these antibodies have been selected for further characterization. One of them have immunological and biochemical characteristics similar to those expected for anchoring filaments. The other has characteristics resembling oxytalan fibers. We have shown that these antigens are synthesized by specific cell populations and have demonstrated the feasibility of their chemical characterization. We propose to isolate and characterize these antigens in detail, to identify the antigen with ultrastructurally observed fibrous components of the basement membrane zone, and to investigate potential interactions between these components and other known connective tissue elements. We anticipate that the proposed studies will provide specific information regarding these basement membrane zone proteins and will help form a basis for the understanding of the structure of this unique region. This information will provide the background for forming hypotheses regarding the molecular events underlying the pathogenesis of epidermolysis bullosa and bullous pemphigoid.

外耳道鳞状上皮下的基底膜 组织在超微结构上比基底膜带更复杂 其他纸巾。除了致密层外，这些区域还包括 附加的细胞成分，如基细胞的半桥粒， 在半桥粒和致密层之间锚定细丝， 锚定的纤维，似乎连接致密层和 潜在的结缔组织，以及可能介导 弹性元件与致密层的相互作用。中国的疾病 大疱性类天疱疮和大疱性表皮松解症影响患者的特定成分 这一区域，并向外部展示这些结构的独特性 纸巾。 作为描述分子网络的第一次尝试， 上皮细胞与深层分子的相互作用 MATRIX，我们已经生产出了针对尚未识别的 组件。这些抗体中的两种已被选择用于进一步 人物刻画。其中一人有免疫学和生化学 类似于锚定细丝的特性。这个 其他的具有类似于奥他兰纤维的特征。我们已经证明了 这些抗原是由特定的细胞群体合成的，并具有 论证了其化学表征的可行性。我们 建议对这些抗原进行详细的分离和鉴定，以鉴定 具有超微结构观察到的纤维成分的抗原 基底膜带，并研究它们之间的潜在相互作用 这些成分和其他已知的结缔组织成分。 我们预计拟议的研究将提供具体的信息。 关于这些基底膜带蛋白，将有助于形成基础 以了解这一独特地区的结构。这 信息将为形成以下假设提供背景 大疱性表皮松解症发病机制的分子事件 和大疱性类天疱疮。