STUDIES OF POLYCYSTIC RENAL DISEASE

本研究的长期目标是定义和表征结构 肾小管基底膜（TBM）的组成部分，从正常动物和 利用结构信息作为阐明潜力的参考 疾病状态的结构缺陷。 我们正在检查几个隧道掘进机 多囊性肾病（PCRD）动物模型的结构异常。 模型包括PCRD，其由二苯胺化学诱导， 二苯基噻唑和天然遗传模型。 可采用的方法有凝胶过滤法、离子交换法、气液法和高压液相色谱法。 高压液相色谱法，分析超离心，电泳， 圆二色光谱和化学分析。

The long-term goals of this study are to define and characterize the structural components of renal tubular basement membrane (TBM) from normal animals and to use the structural information as a reference in elucidating potential structural defects in disease states. We are examining the TBM from several animal models of polycystic renal disease (PCRD) for structural abnormalities. The models include PCRD, which is chemically induced by diphenylamine and diphenylthiazole, and a natural genetic model. The methods to be used include gel filtraton, ion-exchange, gas-liquid, and high pressure liquid chromatography, analytical ultracentrifugation, electrophoresis, circular dichroism spectroscopy, and chemical analyses.