RENAL AMMONIA GENESIS: 15NH3 STUDIES WITH GC-MS

肾氨成因：使用 GC-MS 研究 15NH3

• 批准号: 3153387
• 负责人: ITZHAK NISSIM
• 金额: $ 13.93万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3153387
• 关键词: acidity /alkalinity; alpha ketoglutarate; amides; aminoacid; ammonia; gas chromatography; glutamate dehydrogenase; glutamine; kidney metabolism; mass spectrometry; mitochondria; purine nucleotides; radiotracer; renal cortex; renal tubule

This project proposes to investigate in vitro the sources of urinary ammonia nitrogen. Of particular interest is the possibility that amino acids other than glutamine are important nitrogen donors to renal ammonia, depending upon acid-base status. The influence of pH on ammonia formation from either the amide or alpha nitrogens of glutamine is also a target of study. Emphasis is placed on the respective roles of the purine nucleotide cycle and the glutamate dehydrogenase reactions as metabolic pathways of renal ammoniagenesis. The relationship between ammoniagenesis and the metabolism of alpha-ketoglutarate, particularly the role this organic acid plays in stimulating ammonia formation from glutamate, is another investigative focus. Experiments will be performed in rat kidney cortex slices, isolated renal tubules, kidney mitochondria, and the isolated perfused kidney. A unique feature of the project is the utilization of 15N as a tracer for renal nitrogen metabolism and the utilization of gas chromatography-mass spectrometry to quantitate 15N levels in this tissue. The data so generated will be of scientific value in improving our understanding of renal nitrogen metabolism.

本项目拟在体外研究尿的来源， 氨氮 特别有趣的是氨基 谷氨酰胺以外的酸是肾氨的重要氮供体， 取决于酸碱状态。 pH值对氨生成的影响 来自谷氨酰胺的酰胺或α氮也是 study. 重点放在嘌呤核苷酸各自的作用 循环和谷氨酸脱氢酶反应作为代谢途径 肾氨生成 氨的生成与生物量的关系 α-酮戊二酸的代谢，特别是这种有机酸的作用 在刺激谷氨酸盐形成氨中起作用， 调查重点。 实验将在大鼠肾皮质切片、离体肾皮质切片和离体肾皮质切片中进行。 肾小管、肾线粒体和离体灌注肾。 该项目的一个独特之处是利用15 N作为示踪剂， 肾氮代谢与气相色谱-质谱联用技术 光谱法来定量该组织中的15 N水平。 为止的数据 产生的科学价值，将提高我们的理解， 肾氮代谢

项目成果

Metabolism of glutamine and glutamate by rat renal tubules. Study with 15N and gas chromatography-mass spectrometry.

大鼠肾小管对谷氨酰胺和谷氨酸的代谢。

• 发表时间: 1985
• 期刊: The Journal of biological chemistry
• 作者: Nissim,I;Yudkoff,M;Segal,S
• 通讯作者: Segal,S

Application of gas chromatography-mass spectrometry to in vitro studies of nitrogen metabolism.

气相色谱-质谱联用法在体外氮代谢研究中的应用。

• DOI: 10.1159/000411233
• 发表时间: 1985
• 期刊: Contributions to nephrology
• 作者: Yudkoff,M;Nissim,I;Pleasure,D;Hummeler,K;Segal,S
• 通讯作者: Segal,S

Effect of 5-amino-4-imidazolecarboxamide riboside on renal ammoniagenesis. Study with [15N]aspartate.

5-氨基-4-咪唑甲酰胺核苷对肾产氨的影响。

• 发表时间: 1986
• 期刊: The Journal of biological chemistry
• 作者: Nissim,I;Yudkoff,M;Segal,S
• 通讯作者: Segal,S

Metabolic fate of glutamate carbon in rat renal tubules. Studies with 13C nuclear magnetic resonance and gas chromatography-mass spectrometry.

大鼠肾小管中谷氨酸碳的代谢命运。

• DOI: 10.1042/bj2410361
• 发表时间: 1987
• 期刊: The Biochemical journal
• 作者: Nissim,I;Yudkoff,M;Segal,S
• 通讯作者: Segal,S