MOLECULAR BASIS OF CELLULAR ADHESIVENESS

细胞粘附性是细胞行为的基本特征。 我研究的长期目标是确定 潜在的细胞与细胞外基质的黏附。在.期间 在接下来的五年里，我们打算研究几个一般特征 对粘附性了解甚少，并研究它们之间的关系 纤维连接蛋白受体与其他细胞表面结合部位之间 牵涉到粘连。我们将重点关注的具体问题有 结果如下：(1)我们发现，依赖锰的依恋和 幼年仓鼠肾细胞在培养皿上的铺展 不能用普遍接受的模型解释 细胞黏附。我们计划确定锰对珠子的影响 BHK细胞的结合和吞噬作用及BHK细胞分析 参与锰依赖黏附的成分。(2)我们计划 测定底物结合蛋白去除的特异性 在附着和扩散过程中从局部粘连部位下方 人成纤维细胞及其底物的可能重要性 细胞行为的其他方面的重塑过程。(3)我们 最近已经制备了针对WGA位点的单抗 幼年仓鼠肾脏细胞。似乎与之发生反应的抗体 50kd和140kd的复合纤维连接蛋白受体已经被 部分具有特点，并将进行更详细的研究。我们 计划分析这些受体在细胞黏附中的作用 纤维连接蛋白以外的底物。(4)我们已经分离出一个BHK细胞 细胞与FN涂层培养皿接触有缺陷。我们 将决定这些细胞的FN受体是否相互作用 并分析细胞骨架的其他变化。 细胞成分在细胞-底物接触部位的募集。

Cellular adhesiveness is a fundamental feature of cell behavior. The long-term goal of my research is to determine the mechanisms underlying cell adhesion to extracellular substrata. During the next five years we intent to investigate several general features of adhesion that are poorly understood and to study relationships between fibronectin receptors and other cell surface binding sites involved in adhesion. Specific problems on which we will focus are as follows: (1) We have found that Mn-dependent attachment and spreading of baby hamster kidney (BHK) cells on culture dishes cannot be explained in terms of the generally accepted model of cell adhesion. We plan to determine the effects of Mn on bead binding and phagocytosis by BHK cells and to analyze the BHK cell components involved in Mn-dependent adhesion. (2) We plan to determine the specificity of removal of substratum-bound proteins from beneath focal adhesion sites during attachment and spreading of human fibroblasts and the possible importance of this substratum remodeling process in other aspects of cell behavior. (3) We recently have prepared monoclonal antibodies against the WGA sites of baby hamster kidney cells. Antibodies that appear to react with the 50kd and 140kd complex fibronectin receptors have been partially characterized and will be studied in greater detail. We plan to analyze the role of these receptors in cell adhesion to substrata other than fibronectin. (4) We have isolated a BHK cell variant defective in cell contact to Fn-coated culture dishes. We will determine whether the Fn receptors of these cells interact abnormally with the cytoskeleton and analyze other alterations in recruitment of cell components to sites of cell-substratum contact.