DNA SYNTHESIS: REGULATION IN NORMAL AND CANCER CELLS

这项研究的目的是确定对DNA的可能控制 正常和异常生长过程中的生物合成。大多数DNA聚合酶 从处于S期的HeLa细胞中分离出α活性为MR 640,000 多蛋白形式，命名为DNA聚合酶α2。这种酶有 纯化到电泳均一性，并分解成它的 组成包括：MR 180,000聚合酶α亚基，MR 70,000 DNA引物酶，MR 69,000 3‘-大于5’的核酸外切酶，MR 96,000，C_1和 MR 52,000个C2引物识别蛋白和MR 92,000个AP A结合 蛋白。DNA聚合酶α2与其他必需因子的相互作用 高效、特异地引发RNA引物合成的蛋白质 将会被定义。条件和蛋白质需求除了 DNA链延伸所需的聚合酶α2 将确定催化效率、可加工性和保真度。这个 除了DNA聚合酶α2外，还有酶和非酶蛋白 是SV40-DNA在体外复制所必需的 将对T抗原进行研究。

The objective of the research is to define possible controls for DNA biosynthesis during normal and abnormal growth. Most of the DNA polymerase Alpha activity from HeLa cells in S-phase has been isolated as a Mr 640,000 multiprotein form that is designated DNA polymerase Alpha2. The enzyme has been purified to electrophoretic homogeneity and resolved into its components that include; a Mr 180,000 polymerase Alpha subunit, a Mr 70,000 DNA primase, a Mr 69,000 3'---greater than 5' exonuclease, Mr 96,000 C1 and Mr 52,000 C2 primer-recognition proteins and Mr 92,000 Ap A binding protein. The interaction of DNA polymerase Alpha2 and other required proteins for the efficient and specific initiation of RNA primer synthesis will be defined. The conditions and protein requirements in addition to polymerase Alpha2 that are required for elongation of DNA chains with catalytic efficiency, processivity and fidelity will be ascertained. The enzymic and nonenzymic proteins in addition to DNA polymerase Alpha2 that are required for the replicationof SV40-DNA in vitro in the presence of T-antigen will be studied.