T CELL ABNORMALITIES IN AUTOIMMUNE MRL/1PR

自身免疫 MRL/1PR 中的 T 细胞异常

• 批准号: 3158378
• 负责人: MAN-SUN M SY
• 金额: $ 11.34万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-10-01 至 1990-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3158378
• 关键词: T lymphocyte; antiidiotype antibody; autoimmune disorder; autologous transplantation; cell differentiation; cell population study; clone cells; concanavalin A; discoid lupus erythematosus; disease /disorder model; hybridomas; immunoglobulin idiotypes; laboratory mouse; leukocyte activation /transformation; ligands; mitogens; mixed lymphocyte reaction test; monoclonal antibody; oncogenes

Murine SLE is an appropriate model for human SLE, since most, if not all of the immunological abnormalities fundamental to human SLE can also be readily observe in the murine system. The MHL1pr/1pr mouse is one of the strains that spontaneously develop SLE. In addition, all 1pr/1pr mice develop massive generalized lymph node enlargements as the disease progresses. In these enlarged lymph nodes, more than 90% of the cells are T cells. This project is concerned mainly with the in vitro physiology and immunobiology of the abnormal T cells in 1pr/1pr mice, and the relationship between in vitro T cell abnormalities and development of autoimmunity in vivo. Our research goals are summarized as follows: 1). Characterization of responsiveness of each individual T cell subpopulation in 1pr/1pr mice to receptor driven activation signals or polyclonal activators. 2). Characterization of the consequence of activation in vitro with respect to cell surface phenotypes and their differentiation state. Correlation between in vitro observations and in vivo pathogenesis. 3) Characterization of immune abnormalities in vitro and the establishment of an in vitro model system for the study of differentiation of 1pr/1pr T cells.

小鼠系统性红斑狼疮是人类系统性红斑狼疮的合适模型，因为大多数，如果 并不是所有人类基本的免疫异常 在小鼠体内也很容易观察到系统性红斑狼疮。这个 MHL1Pr/1PR小鼠是一种自发的 发展系统性红斑狼疮。此外，所有1PR/1PR小鼠都发展成大量 随着疾病的进展，全身性的淋巴结肿大。 在这些增大的淋巴结中，90%以上的细胞是T细胞 细胞。这个项目主要与体外培养有关。 1PR/1PR中异常T细胞的生理和免疫生物学研究 小鼠，以及体外T细胞异常之间的关系 和体内自身免疫的发展。我们的研究目标是 摘要如下： 1)。每个单独T细胞的反应性特征 1PR/1PR小鼠受体驱动激活的亚群 信号或多克隆激活剂。 2)。体外激活后果的表征 关于细胞表面表型和它们的 分化状态。体外试验中的相关性 观察和体内发病机制。 3)体外免疫异常的特征和 一种体外模型系统的建立及其应用 1PR/1PR T细胞的分化。