ELECTRICAL AND CONTRACTILE PROPERTIES OF MUSCLE

当骨骼肌纤维去极化到收缩的电位时 激活显示一个小的，瞬时的外向电流，这可以归因于 限制在膜上的电荷的运动或重新排列。这个 这种与电压有关的电荷运动的特征表明，它可能 起到激活收缩的作用。计划进行实验，以比较一些 这些电荷运动的特性与钙离子释放的特性 事实上，这两个过程是否相关。如果是这样的话，我们将努力找到 弄清楚你们的关系是什么。 指示剂染料将被用来估计细胞内的pH和镁离子 并观察染料在肌肉内的表现是否有所不同 纤维，因为结合，而不是在试管中。 人们将尝试合成新的更好的钙离子指示剂。 这些需要与简单化学计量比的钙离子形成络合物， 对pH和镁的变化不敏感，速度快，不与细胞结合 选民。将进行搜索，以找到一种对 钙离子，以便在抽动过程中释放的大多数钙离子将结合到 染料。来自这种染料的光信号将被用来研究时间过程 而不是电离钙离子的时间过程。

Skeletal muscle fibers when depolarized to a potential at which contraction is activated show a small, transient outward current which can be attributed to movement or rearrangement of charges confined to the membrane. The characteristics of this voltage dependent charge movement suggest that it may play a role in activating contraction. Experiments are planned to compare some of the properties of these charge movements with those of Ca ion release to see whether, in fact, the two processes are related. If so, we shall try to find out what the relationship is. Indicator dyes will be used to obtain estimates of intracellular pH and Mg ions in muscle fibers and to see whether dyes behave differently inside muscle fibers, because of binding, than they do in cuvettes. An attempt will be made to synthesize new and better Ca ion indicator dyes. These need to form complexes with Ca ions of simple stoichiometry, to be insensitive to changes in pH and Mg, to be fast, and to not bind to cellular constituents. A search will be made to find a dye that has a high affinity for Ca ions so that most of the Ca ions released during a twitch would be bound to the dye. Optical signals from this dye would be used to study the time course of Ca ion release rather than the time course of ionized Ca ions.