ELECTRICAL AND CONTRACTILE PROPERTIES OF MUSCLE

骨骼肌纤维去极化到收缩电位时， 激活显示出一个小的，瞬态外向电流，这可以归因于 限制在膜上的电荷的移动或重新排列。 的 这种依赖于电压的电荷运动的特性表明， 起到激活收缩的作用。 实验计划比较一些 这些电荷运动的性质与钙离子释放的性质， 事实上，这两个过程是否相关。 如果是这样，我们将试图找到 他们之间的关系是什么 指示染料将用于获得细胞内pH和Mg离子的估计值 并观察染料在肌肉中的表现是否不同 纤维，因为结合，比他们在比色皿中。 本文试图合成新的、性能更好的钙离子指示染料。 这些需要与简单化学计量的Ca离子形成络合物， 对pH和Mg的变化不敏感，快速，并且不与细胞结合， 选民。 将进行搜索以找到对以下具有高亲和力的染料： 因此，在抽搐过程中释放的大部分钙离子将被束缚在 染料 来自这种染料的光学信号将用于研究时间过程 钙离子释放的时间过程，而不是电离钙离子的时间过程。

Skeletal muscle fibers when depolarized to a potential at which contraction is activated show a small, transient outward current which can be attributed to movement or rearrangement of charges confined to the membrane. The characteristics of this voltage dependent charge movement suggest that it may play a role in activating contraction. Experiments are planned to compare some of the properties of these charge movements with those of Ca ion release to see whether, in fact, the two processes are related. If so, we shall try to find out what the relationship is. Indicator dyes will be used to obtain estimates of intracellular pH and Mg ions in muscle fibers and to see whether dyes behave differently inside muscle fibers, because of binding, than they do in cuvettes. An attempt will be made to synthesize new and better Ca ion indicator dyes. These need to form complexes with Ca ions of simple stoichiometry, to be insensitive to changes in pH and Mg, to be fast, and to not bind to cellular constituents. A search will be made to find a dye that has a high affinity for Ca ions so that most of the Ca ions released during a twitch would be bound to the dye. Optical signals from this dye would be used to study the time course of Ca ion release rather than the time course of ionized Ca ions.