CONTROL MODEL FOR HEALING ACROSS A BONE CHAMBER IMPLANT

这个项目的主要目标是建立一个定量的伤口 修复性成骨、新血管形成模式的愈合模型 以及再生成骨腔的组织盘中的微循环 植入兔胫骨内侧皮质骨 高原 将对修复过程的长期观察进行分析， 这个组织和再生成兔子的组织之间的差异 耳腔 该项目的长期目标是 用损伤扰乱这种伤口愈合模型， 新血管形成和微循环足以诱导 病理生理反应的发展和逆转， 进行观察和定量分析。 在未来的项目中， 将作为研究缺血，感染， 各种化学和电学试剂等对骨修复的影响。 实现所有目标的主要工具是横向 活体显微镜和长期植入的经皮质骨 室。 规范模型的一般工作假设是， 骨腔的缝隙： 1)血管的生长模式与耳室内的相似， 骨长入开始。 2)随着再生骨的进展，血管的数量和体积 在前进的前锋减少，而相同的数量内， 骨增加。 3)体积流速在骨坏死前一周达到最大值， 向内生长 4)主要血管将倾向于与血管的长轴平行排列。 骨长入开始后的主骨;即使没有哈弗氏管 form. 5)在骨前缘发育过程中，编织骨先于板层骨。 假设检验将从胫骨中植入骨腔开始开始。 术后一周，将植入的腿安装在轭架上，以便对齐 腔室光轴与活体成像的光轴 显微镜 将记录狭缝视野中的愈合组织 录像带和静态照片，利用简单和偏振 透射照明。 流型观察将包括FITC-葡聚糖 在漫射激光落射照明下的荧光。 意见将 每周持续一次，直至术后第七周。 将使用图像分析系统进行分析，该系统包括 视频位置分析器、帧存储器、微机和互相关 速度计

The primary goal of this project is to establish a quantitative wound healing model for patterns of reparative osteogenesis, neovascularization and microcirculation in a disc of tissue regenerating into a bone chamber which has been implanted in the cortical bone of rabbit tibia medial plateau. Chronic observation of the repair process will be analyzed for differences between this tissue and that which regenerates into the rabbit ear chamber. The long term objective which will follow this project is to perturb this wound healing model with insults which will alter cortical neovascularization and microcirculation sufficiently to induce a pathophysiological response to the development and reversal of which will be observed and quantitatively analyzed. In future projects this model will serve as a baseline for studies of the effects of ischemia, infection, various chemical and electrical agents, etc. on bone repair. The primary tools for achieving all objectives are the horizontal intravital microscope and the chronically implanted transcortical bone chamber. The general working hypotheses for the norm model are that in the slit-gap of the bone chamber: 1) The vascular growth pattern is similar to that in the ear chamber until bony ingrowth commences. 2) As the regenerating bone advances the number and volume of vessels before the advancing front decrease while the same quantities within the bone increase. 3) Volumetric flow rates reach a maximum during the week preceding bony ingrowth. 4) Major blood vessels will tend to align parallel with the long axis of the main bone after bony ingrowth commences; even if no haversian canals form. 5) Woven bone precedes lamellar bone during bone front development. Hypothesis testing will begin by implanting the bone chamber in the tibia. One week post-op the implanted leg will be mounted in a yoke so as to align the chamber optical axis with the optical axis of an intravital microscope. The healing tissue in the slit field will be recorded on videotape and still photographs, utilizing simple and polarized trans-illumination. Flow pattern observations will include FITC-Dextran fluorescence under diffused laser epi-illumination. Observations will continue weekly through the seventh post-operative week. Analysis will be performed with an image analysis system which includes a video position analyzer, frame memory, microcomputer and cross-correlation velocimeter.