CONTROL MODEL FOR HEALING ACROSS A BONE CHAMBER IMPLANT

骨腔植入物愈合的控制模型

• 批准号: 3157206
• 负责人: HOWARD WINET
• 金额: $ 15.74万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-04-01 至 1989-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3157206
• 关键词: angiogenesis; bone necrosis; bone regeneration; disease /disorder model; implant; microcirculation; microscopy; osteogenesis; wound healing

The primary goal of this project is to establish a quantitative wound healing model for patterns of reparative osteogenesis, neovascularization and microcirculation in a disc of tissue regenerating into a bone chamber which has been implanted in the cortical bone of rabbit tibia medial plateau. Chronic observation of the repair process will be analyzed for differences between this tissue and that which regenerates into the rabbit ear chamber. The long term objective which will follow this project is to perturb this wound healing model with insults which will alter cortical neovascularization and microcirculation sufficiently to induce a pathophysiological response to the development and reversal of which will be observed and quantitatively analyzed. In future projects this model will serve as a baseline for studies of the effects of ischemia, infection, various chemical and electrical agents, etc. on bone repair. The primary tools for achieving all objectives are the horizontal intravital microscope and the chronically implanted transcortical bone chamber. The general working hypotheses for the norm model are that in the slit-gap of the bone chamber: 1) The vascular growth pattern is similar to that in the ear chamber until bony ingrowth commences. 2) As the regenerating bone advances the number and volume of vessels before the advancing front decrease while the same quantities within the bone increase. 3) Volumetric flow rates reach a maximum during the week preceding bony ingrowth. 4) Major blood vessels will tend to align parallel with the long axis of the main bone after bony ingrowth commences; even if no haversian canals form. 5) Woven bone precedes lamellar bone during bone front development. Hypothesis testing will begin by implanting the bone chamber in the tibia. One week post-op the implanted leg will be mounted in a yoke so as to align the chamber optical axis with the optical axis of an intravital microscope. The healing tissue in the slit field will be recorded on videotape and still photographs, utilizing simple and polarized trans-illumination. Flow pattern observations will include FITC-Dextran fluorescence under diffused laser epi-illumination. Observations will continue weekly through the seventh post-operative week. Analysis will be performed with an image analysis system which includes a video position analyzer, frame memory, microcomputer and cross-correlation velocimeter.

该项目的主要目标是建立一个量化伤口 修复性成骨、新血管形成模式的愈合模型 以及再生成骨室的组织盘中的微循环 已植入兔胫骨内侧皮质骨 高原。 修复过程的长期观察将被分析 该组织与兔子再生的组织之间的差异 耳室。 该项目的长期目标是 用侮辱扰乱这个伤口愈合模型，这会改变皮质 新血管形成和微循环足以诱导 病理生理反应的发展和逆转将 进行观察和定量分析。 在未来的项目中这个模型 将作为研究缺血、感染、 各种化学剂和电剂等对骨修复的作用。 实现所有目标的主要工具是横向 活体显微镜和长期植入的经皮质骨 室。 范数模型的一般工作假设是 骨室的狭缝间隙： 1) 血管生长模式与耳腔相似，直到 骨质开始向内生长。 2）随着骨的再生，血管的数量和体积不断增加 在前进锋之前减少，而在前进锋内相同的数量 骨质增加。 3) 体积流量在骨形成前一周达到最大值 向内生长。 4）主要血管倾向于与长轴平行排列 骨向内生长开始后的主骨；即使没有哈弗运河 形式。 5) 在骨前端发育过程中，编织骨先于板层骨。 假设检验将从将骨室植入胫骨开始。 术后一周，植入的腿将安装在轭架中，以便对齐 腔室光轴与活体光轴 显微镜。 狭缝区域中的愈合组织将被记录在 录像带和静态照片，利用简单和偏光 透射照明。 流型观察将包括 FITC-葡聚糖 漫射激光落射照明下的荧光。 观察将 每周持续至术后第七周。 将使用图像分析系统进行分析，该系统包括 视频位置分析仪、帧存储器、微机和互相关 测速仪。