CONTROL MODEL FOR HEALING ACROSS A BONE CHAMBER IMPLANT

这个项目的主要目标是建立一个量化创伤 修复性成骨、新生血管模式的愈合模型 以及再生成骨腔的组织盘中的微循环 将其植入兔胫骨内侧皮质骨。 平台期。对修复过程的长期观察将分析 这种组织与再生成兔的组织的区别 耳腔。该项目之后的长期目标是 用侮辱扰乱这个伤口愈合模型，这会改变大脑皮质 新生血管和微循环足以诱导 对其发生和逆转的病理生理反应 被观察和定量分析。在未来的项目中，这种模式 将作为研究缺血、感染、 各种化学、电剂等对骨修复的作用。 实现所有目标的主要工具是水平 活体显微镜和长期植入的经皮质骨 密室。范数模型的一般工作假设是在 骨室的缝隙： 1)血管生长模式与耳室相似，直到 骨质疏松症开始。 2)随着新生骨的推进，血管的数量和体积 在前进锋面减小之前，而在 骨量增加。 3)体积流量在骨骼前一周达到最大值 内生性。 4)主要血管倾向于与血管的长轴平行排列 骨长入后的主骨开始生长；即使没有哈弗斯管 形式。 5)在骨锋发育过程中，编织骨先于板层骨发育。 假说检验将从将骨腔植入胫骨开始。 术后一周，植入的小腿将被安装在一个轭上，以便对齐 舱内光轴与活体的光轴 显微镜。缝隙中的愈合组织将被记录在 录像带和静态照片，利用简单和极化 变光。流型观测将包括FITC-葡聚糖 漫射激光表面光下的荧光。观察将会 继续每周一次，直到术后第七周。 分析将使用图像分析系统进行，该系统包括 视频位置分析器、帧存储器、微型计算机和互相关 测速仪。

The primary goal of this project is to establish a quantitative wound healing model for patterns of reparative osteogenesis, neovascularization and microcirculation in a disc of tissue regenerating into a bone chamber which has been implanted in the cortical bone of rabbit tibia medial plateau. Chronic observation of the repair process will be analyzed for differences between this tissue and that which regenerates into the rabbit ear chamber. The long term objective which will follow this project is to perturb this wound healing model with insults which will alter cortical neovascularization and microcirculation sufficiently to induce a pathophysiological response to the development and reversal of which will be observed and quantitatively analyzed. In future projects this model will serve as a baseline for studies of the effects of ischemia, infection, various chemical and electrical agents, etc. on bone repair. The primary tools for achieving all objectives are the horizontal intravital microscope and the chronically implanted transcortical bone chamber. The general working hypotheses for the norm model are that in the slit-gap of the bone chamber: 1) The vascular growth pattern is similar to that in the ear chamber until bony ingrowth commences. 2) As the regenerating bone advances the number and volume of vessels before the advancing front decrease while the same quantities within the bone increase. 3) Volumetric flow rates reach a maximum during the week preceding bony ingrowth. 4) Major blood vessels will tend to align parallel with the long axis of the main bone after bony ingrowth commences; even if no haversian canals form. 5) Woven bone precedes lamellar bone during bone front development. Hypothesis testing will begin by implanting the bone chamber in the tibia. One week post-op the implanted leg will be mounted in a yoke so as to align the chamber optical axis with the optical axis of an intravital microscope. The healing tissue in the slit field will be recorded on videotape and still photographs, utilizing simple and polarized trans-illumination. Flow pattern observations will include FITC-Dextran fluorescence under diffused laser epi-illumination. Observations will continue weekly through the seventh post-operative week. Analysis will be performed with an image analysis system which includes a video position analyzer, frame memory, microcomputer and cross-correlation velocimeter.