STUDIES OF FIBRIN DEPOSITION IN CANCER

癌症中纤维蛋白沉积的研究

• 批准号: 3165767
• 负责人: FREDERICK R. RICKLES
• 金额: $ 11.48万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-07-01 至 1988-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3165767
• 关键词: anticoagulants; cytotoxicity; disease /disorder model; fibrin; fibrinolysis; human subject; human therapy evaluation; immune complex; monoclonal antibody; neoplasm /cancer chemotherapy; neoplasm /cancer immunology; neoplasm /cancer invasiveness; neoplastic cell; peptides; radioimmunoassay; tissue /cell culture; warfarin

This research proposal seeks information about the mechanism(s) for fibrin deposition in cancer. Such information is critical for the design of treatment regimens aimed at this host defense mechanism. The proposal focuses on developing an understanding of the relative importance of tumor-associated procoagulants (PCA) and macrophage tissue factor (MO-TF) in this process. There are four specific goals: 1. Identification of antigens related to tissue factor (TF) (TF:RAg), other tumor PCA, and fibrin in human tumors in situ. 2. Identification and molecular characterization of TF (and other PCA) in isolated human tumor cells and tumor-associated MO. 3. Production and characterization of monoclonal antibodies to rabbit TF. 4. Determination of the in vivo effects of TF-specific, monoclonal antibodies on the expression of MO-TF, the deposition of fibrin and tumor growth in a rabbit tumor model. (A)

这项研究计划旨在寻求有关纤维蛋白的机制的信息， 沉积在癌症中。 这些信息对于设计 针对这种宿主防御机制的治疗方案。 该提案 重点是了解以下方面的相对重要性： 肿瘤相关促凝剂（PCA）和巨噬细胞组织因子（MO-TF） 在这个过程中。 具体目标有四个： 1. 组织因子（TF）相关抗原（TF：RAg）的鉴定， 其他肿瘤PCA和人原位肿瘤中的纤维蛋白。 2. TF（和其他PCA）的鉴定和分子表征 分离的人肿瘤细胞和肿瘤相关MO。 3. 兔TF单克隆抗体的制备及鉴定。 4. TF特异性单克隆抗体的体内作用的测定 抗体对MO-TF表达、纤维蛋白沉积和 在兔肿瘤模型中的肿瘤生长。 （一）