ROLES OF LAMININ AND ENTACTIN IN CELL ADHESION

层粘连蛋白和巢蛋白及基底的主要成分 膜。 有充分的证据表明，层粘连蛋白介导的细胞凋亡是一个重要的过程。 粘附和扩散、神经突生长和神经元存活。 也有证据表明，它参与轴突， 指导，神经肌肉接头的形成，肾 发育、胚胎发生和维持分化的 功能协调发展的 层粘连蛋白及其受体的相互作用已被广泛研究。 与转移过程和识别 自然杀伤淋巴细胞及其靶细胞。 的 巢蛋白参与基底膜的功能， 不太清楚。 发现它与层粘连蛋白紧密非共价结合 最近的证据表明，它也可能参与细胞 附件. 本建议的目标是：（a）确定 层粘连蛋白是细胞附着所必需的。 (b)分离和 表征介导层粘连蛋白的细胞表面分子 结合，（c）阐明巢蛋白在基底膜中的作用 特别是在细胞附着方面起作用，以及（d） 探讨层粘连蛋白结合蛋白的表达调控 大分子 层粘连蛋白和巢蛋白的一级序列 将通过重组DNA技术确定。 融合 将产生含有每个分子片段的蛋白质 用作细胞结合的探针和抗原。 多克隆 和用所述融合蛋白产生的单克隆抗体， 将使用源自层粘连蛋白和巢蛋白的多肽 以阐明它们的绑定域。 长期目标： 该项目旨在确定细胞外 基质影响细胞在转移过程中的行为， 胚胎发生和细胞分化。

Laminin and entactin and major components of basement membranes. It is well documented that laminin mediated cell adhesion and spreading, neurite outgrowth and neuronal survival. There is also evidence to suggest that it is involved in axonal guidance, formation of neuromuscular junctions, kidney development, embryogenesis and maintenance of differentiated functions. The interactions of laminin and its receptor has been implicated in the metastatic process and in the recognition of natural killer lymphocytes and their target cells. The involvement of entactin in the function of basement membranes is less clear. It found in tight non-covalent association with laminin and recent evidence suggests that it may also be involved in cell attachment. The objectives of this proposal are to (a) define the structures in laminin that are required for cell attachment. (b) isolate and characterize the cell surface molecules the mediate laminin binding, (c) clarify the role of entactin in basement membrane function especially with respect to cell attachment and (d) explore the regulation of expression of the laminin binding macromolecules. The primary sequences of laminin and entactin will be determined by recombinant DNA techniques. Fusion proteins containing segments of each molecule will be generated to be used as probes for cell binding and as antigens. Polyclonal and monoclonal antibodies produced with the fusion proteins and polypeptides derived from laminin and entactin will be employed to eluctidate their bindings domains. The long range objective of the project is to define the mechanisms whereby the extracellular matrix influences the behavior of cells during metastasis, embryogenesis and cellular differentiation.