CHARACTERIZATION OF BASEMENT MEMBRANE GLYCOPROTEINS

这项建议的长期目标是了解该组织 基底膜及其在正常和病理状态下的作用。 基底膜是复杂的细胞外基质，参与了 组织组织、胚胎发生、细胞黏附、过滤和 保护。许多患者的基底膜结构都有改变。 糖尿病、癌症、大疱性类天疱疮等病理状态， 哮喘、不同来源的肾小球肾炎和几种自身免疫性疾病 疾病。这项建议的具体目标有两个：(A) 将用产生的模型矩阵来研究基底膜组织 小鼠内胚层细胞的细胞培养和(B)基因的调控 对于层粘连蛋白和牙本质蛋白，将有两个基底膜成分 探索过了。将采用的技术包括建造 杂交瘤细胞产生检测基底膜的特异性探针 组织，肽分离和测序，分离和 层粘连蛋白和层粘连蛋白基因编码DNA序列的特征 Entactin和维甲酸对胚胎癌细胞F9的诱导作用 和二丁酰环AMP，研究层粘连蛋白和粘连蛋白的表达。

The long term objective of this proposal is to understand the organization of basement membranes and their role in normal and pathological states. Basement membranes are complex extracellular matrices that are involved in tissue organization, embryogenesis, cell adhesion, filtration and protection. There are alterations in basement membrane structures in many pathological states such as diabetes mellitus, cancer, bullous pemphigoid, asthma, glomeruluo-nephritis of varied origin and several of the autoimmune diseases. The specific objectives of this proposal are two fold: (a) basement membrane organization will be studied with a model matrix produced in cell culture by mouse endoderm cells and (b) the regulation of the genes for laminin and entactin, two basement membrane components will be explored. The techniques that will be employed include the construction of hybridoma cells to generate specific probes for examining basement membrane organization, peptide isolation and sequencing, isolation and characterization of DNA sequences that code for the genes of laminin and entactin, and induction of embryonal carcinoma F9 cells with retinoic acid and dibutyryl cyclic AMP to study the expression of laminin and entactin.