MOLECULAR BASIS OF CELLULAR ADHESIVENESS

细胞周期是细胞行为的基本特征。 我研究的长期目标是确定 潜在的细胞粘附到细胞外基质。 期间 在接下来的五年里，我们打算研究几个一般特征， 我们对粘附力的了解还很少， 纤连蛋白受体和其他细胞表面结合位点之间的相互作用 参与粘附。 我们将重点关注的具体问题是 如下：（1）我们已经发现，锰依赖性的连接和 幼仓鼠肾（BHK）细胞在培养皿上的铺展 不能用普遍接受的模型来解释， 细胞粘附 我们计划确定锰对珠粒的影响， BHK细胞的结合和吞噬作用，并分析BHK细胞 参与Mn依赖性粘附的组分。(2)我们计划 确定去除基质结合蛋白的特异性 在附着和展开过程中， 以及这种基质可能的重要性 在细胞行为的其他方面重塑过程。(3)我们 最近已经制备了针对WGA位点的单克隆抗体 仓鼠幼仔肾细胞 抗体似乎与 50 kD和140 kD复合纤连蛋白受体已被 部分特征，并将进行更详细的研究。 我们 计划分析这些受体在细胞粘附中的作用， 除了纤连蛋白以外的基质。(4)我们分离出一个BHK细胞 在与Fn包被的培养皿的细胞接触中有缺陷的变体。 我们 将决定这些细胞的Fn受体是否 与细胞骨架异常，并分析其他变化， 将细胞组分募集到细胞-基质接触的位点。

Cellular adhesiveness is a fundamental feature of cell behavior. The long-term goal of my research is to determine the mechanisms underlying cell adhesion to extracellular substrata. During the next five years we intent to investigate several general features of adhesion that are poorly understood and to study relationships between fibronectin receptors and other cell surface binding sites involved in adhesion. Specific problems on which we will focus are as follows: (1) We have found that Mn-dependent attachment and spreading of baby hamster kidney (BHK) cells on culture dishes cannot be explained in terms of the generally accepted model of cell adhesion. We plan to determine the effects of Mn on bead binding and phagocytosis by BHK cells and to analyze the BHK cell components involved in Mn-dependent adhesion. (2) We plan to determine the specificity of removal of substratum-bound proteins from beneath focal adhesion sites during attachment and spreading of human fibroblasts and the possible importance of this substratum remodeling process in other aspects of cell behavior. (3) We recently have prepared monoclonal antibodies against the WGA sites of baby hamster kidney cells. Antibodies that appear to react with the 50kd and 140kd complex fibronectin receptors have been partially characterized and will be studied in greater detail. We plan to analyze the role of these receptors in cell adhesion to substrata other than fibronectin. (4) We have isolated a BHK cell variant defective in cell contact to Fn-coated culture dishes. We will determine whether the Fn receptors of these cells interact abnormally with the cytoskeleton and analyze other alterations in recruitment of cell components to sites of cell-substratum contact.