ANTIGEN MARKERS IN DIAGNOSIS OF PROSTATE CANCER

这项建议的目的是继续我们对抗原标志物的研究， 可以用于检测前列腺癌。 一个新的，具体的， 酸性磷酸酶的灵敏固相免疫荧光测定法是 评价其在前列腺癌早期诊断中的潜在价值。 一 前列腺特异性抗原已被纯化至同质。 异种抗血清具有 针对前列腺抗原和抗血清的特异性 将被确定。 初步数据表明，该抗原可能具有潜在的 用于鉴别前列腺癌患者。 一种新的灵敏的免疫测定法， 如放射免疫测定法或酶免疫测定法，将被开发和评估。 在 此外，前列腺肿瘤特异性抗原已被证明， 在非人类灵长类动物（狒狒）中产生了针对它的抗体。 孤立， 该前列腺癌抗原的纯化和表征将是 执行。 此外，前列腺酸性磷酸酶结合球蛋白已被发现。 通过使用纯化的亲和色谱法从正常人血清中分离 前列腺酸性磷酸酶 其在前列腺的潜在临床应用 将研究肿瘤放射定位和免疫治疗。 最后通过一个 用于测量血清核糖核酸酶的免疫测定法正在被开发， 已经实现了两种前列腺核糖核酸酶的纯化。

The objective of this proposal is to continue our study on antigen markers which may be useful in the detection of prostate cancer. A new, specific and sensitive solid-phase immunofluorescence assay for acid phosphatase is to be evaluated for its potential value in the early diagnosis of prostate cancer. A prostate-specific antigen has been purified to homogeneity. Xenoantisera have been raised against the prostate antigen and the specificity of the antisera will be determined. Initial data indicate that this antigen may be of potential use in identifying prostate cancer patients. A new sensitive immunoassay, such as radioimmunoassay or enzyme immunoassay, will be developed and evaluated. In addition, a prostate tumor-specific antigen has been demonstrated and antiserum against it has been produced in non-human primates (baboon). Isolation, purification and characterization of this prostate cancer antigen will be performed. Furthermore, a prostate acid phosphatase-binding globulin has been isolated from normal human serum by affinity chromatography using purified prostatic acid phosphatase. Its potential clinical application in prostate tumor radiolocalization and immunotherapy will be studied. Finally, an immunoassay for measurement of serum ribonuclease is being developed, as purification of two prostatic ribonucleases has been achieved.