In vivo alpha-olefin production: a sustainable hydrocarbon source

体内α-烯烃生产：可持续的碳氢化合物来源

• 批准号: BB/K017802/1
• 负责人: David Leys
• 金额: $ 77.27万
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FK017802%2F1
• 关键词: vivo; alpha; olefin; production; sustainable

One of the main challenges our society faces is the dwindling level of oil reserves which we not only depend upon for transport fuels, but also plastics, lubricants and a wide range of petrochemicals. This application seeks to provide an answer through synthetic biology: making organisms produce "oil". Although the production of oil is mainly a biogenic process, the geochemical conversion of biological matter to oil occurs over thousands of years. Solutions that seek to reduce our dependency on fossil oil are therefore urgently needed. The direct production of hydrocarbon compounds by living organisms, bypassing the geochemical conversion of organic matter into oil, is an attractive process, but is unfortunately not part of the "mainstream" repertoire of biochemical reactions that would be required to make this an attractive sustainable alternative. Indeed, minor pathways or side-reactions resulting in the production of hydrocarbons such as alkenes or alkanes have only recently been documented. Unfortunately, these are not present in any organism to the scale and/or specificity that would support industrial application, let alone provide a valid alternative to fossil oil. However, the application of synthetic biology and metabolic engineering to modify these pathways is likely to result in innovative advances in this area. To meet these challenges, we will combine state-of-the-art enzymology and laboratory evolution techniques with synthetic biology. The first challenge is enzymatic hydrocarbon production, a process that often starts with fatty acids. The enzymes involved in converting fatty acids to hydrocarbons have only very recently been identified and many remain unstudied. Furthermore, their properties (substrate and product specificity, stability and rate) are unlikely to support an industrial scale process. We will investigate the use of a wide range of enzymes using structure-based rational engineering and laboratory evolution, in order to create a comprehensive toolkit of catalysts that we will exploit for hydrocarbon production. Ultimately, we will attempt to integrate these with various components into a bacterial strain which can convert renewables into hydrocarbons, preferentially excreted to the outside environment, creating a sustainable process. This ambitious programme addresses an urgent industrial need for reducing our dependency on fossil oil. Through enzyme design and development of new pathways, it will generate "oil"-producing organisms, hence bypassing the need to drastically adapt oil-dependent processes while reducing the associated carbon footprint. Our project will focuses in particular on production of linear alpha-olefins, a high value, industrially crucial intermediate class of hydrocarbons that are key chemical intermediates in a variety of applications, such as flexible packaging, rigid packaging and pipes, synthetic lubricants used in passenger car, heavy duty motor and gear oils, surfactants, detergents, lubricant additives and paper sizing. At present, no "green" alpha-olefin production process is available, a situation which this application seeks to change.

我们社会面临的主要挑战之一是石油储量的减少，我们不仅依赖于运输燃料，而且还依赖于塑料，润滑剂和各种石化产品。这一应用试图通过合成生物学提供答案：让生物体产生“油”。虽然石油的生产主要是一个生物过程，但生物物质向石油的地球化学转化发生了数千年。因此，迫切需要寻求减少我们对化石石油依赖的解决方案。由生物体直接生产碳氢化合物，绕过有机物质转化为石油的地球化学转化，是一个有吸引力的过程，但不幸的是，这不是使其成为一个有吸引力的可持续替代方法所需的“主流”生化反应的一部分。事实上，导致烃如烯烃或烷烃的生产的次要途径或副反应最近才被记录。不幸的是，这些都不存在于任何生物体的规模和/或特异性，将支持工业应用，更不用说提供一个有效的替代化石石油。然而，应用合成生物学和代谢工程来改变这些途径可能会导致这一领域的创新性进展。为了迎接这些挑战，我们将联合收割机结合最先进的酶学和实验室进化技术与合成生物学。第一个挑战是酶促碳氢化合物生产，这是一个通常从脂肪酸开始的过程。参与将脂肪酸转化为碳氢化合物的酶只是最近才被鉴定出来，许多酶尚未研究。此外，它们的性质（底物和产物特异性、稳定性和速率）不太可能支持工业规模工艺。我们将使用基于结构的合理工程和实验室进化来研究各种酶的使用，以创建一个全面的催化剂工具包，我们将利用它来生产碳氢化合物。最终，我们将尝试将这些与各种成分整合到一种细菌菌株中，这种菌株可以将可再生能源转化为碳氢化合物，优先排出到外部环境中，从而创造一个可持续的过程。这一雄心勃勃的方案满足了减少我们对化石石油依赖的迫切工业需要。通过酶的设计和新途径的开发，它将产生“石油”生产生物体，从而绕过需要大幅调整依赖石油的过程，同时减少相关的碳足迹。我们的项目将特别关注线性α-烯烃的生产，这是一种高价值的工业关键中间烃类，是各种应用中的关键化学中间体，如软包装，硬包装和管道，乘用车中使用的合成润滑剂，重型电机和齿轮油，表面活性剂，洗涤剂，润滑剂添加剂和纸张施胶剂。目前，没有“绿色”α-烯烃生产方法可用，这是本申请寻求改变的情况。

项目成果

Ion Mobility Mass Spectrometry Measures the Conformational Landscape of p27 and its Domains and how this is Modulated upon Interaction with Cdk2/cyclin A

离子淌度质谱法测量 p27 及其结构域的构象景观，以及如何在与 Cdk2/cyclin A 相互作用时对其进行调节

• DOI: 10.1002/ange.201812697
• 发表时间: 2019
• 期刊: Angewandte Chemie
• 作者: Beveridge R

Using Native Mass Spectrometry to Analyse Proteins Directly from Food

使用天然质谱法直接分析食物中的蛋白质

• DOI: 10.26434/chemrxiv-2023-spndb
• 发表时间: 2023
• 作者: France A

Mass spectrometry locates local and allosteric conformational changes that occur on cofactor binding.

• DOI: 10.1038/ncomms12163
• 发表时间: 2016-07-15
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Beveridge R;Migas LG;Payne KAP;Scrutton NS;Leys D;Barran PE
• 通讯作者: Barran PE

The role of conserved residues in Fdc decarboxylase in prenylated flavin mononucleotide oxidative maturation, cofactor isomerization, and catalysis.

• DOI: 10.1074/jbc.ra117.000881
• 发表时间: 2018-02-16
• 期刊: The Journal of biological chemistry
• 作者: Bailey SS;Payne KAP;Fisher K;Marshall SA;Cliff MJ;Spiess R;Parker DA;Rigby SEJ;Leys D
• 通讯作者: Leys D