AVIAN SARCOMA VIRUS TUMOR ANTIGENS

禽肉瘤病毒肿瘤抗原

• 批准号: 3168594
• 负责人: J THOMAS PARSONS
• 金额: $ 14.47万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-01-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3168594
• 关键词: Retroviridae disease; Rous sarcoma virus; antiserum; autoradiography; cell growth regulation; enzyme induction /repression; enzyme mechanism; gel electrophoresis; genetic manipulation; genetic mapping; hybridomas; immunochemistry; immunogenetics; laboratory mouse; laboratory rabbit; microinjections; molecular oncology; monoclonal antibody; oncogenes; oncogenic virus; oncoproteins; phosphorylation; protein sequence; protein tyrosine kinase; radioimmunoassay; tissue /cell culture; transfection; tumor antigens; viral carcinogenesis; virus protein

Genetic and biochemical experiments have shown that the product of the Rous sarcoma virus src gene is required for the initiation and maintenance of cellular transformation. As a part of our long-term goal of understanding how the src gene product, pp60src, mediates the molecular events involved in cellular transformation, we have isolated and partially characterized monoclonal antibodies directed against pp60src. The experiments outlined in this proposal employ monoclonal antibodies to unique determinants within pp60src as immunochemical probes to define and characterize the structural and functional domains of the molecule and attempt to elucidate the role of individual functional domains in mediating cellular transformation. We further propose to utilize monoclonal antibodies to pp60src as immunochemical probes to compare the structure and function of other retrovirus tyrosine protein kinase as well as normal cell tyrosine protein kinases. These studies will permit us to define the structural and functional domains of pp60src and allow us to relate this information to other proteins which may be members of a family of related tyrosine protein kinases.

遗传和生化实验表明，Rous的产物 肉瘤病毒src基因在肿瘤的启动和维持中是必需的 细胞转化。作为我们理解的长期目标的一部分 Src基因产物pp60src如何调节相关的分子事件 在细胞转化中，我们分离并部分表征了 针对pp60src的单抗。概述了这些实验 在这项建议中，使用了针对特定决定因素的单抗 Pp60src作为免疫化学探针来定义和表征结构 和分子的功能结构域，并试图阐明其作用 调节细胞转化的单个功能结构域。我们 进一步建议利用pp60src的单抗作为 免疫化学探针法比较其他基因的结构和功能 逆转录病毒酪氨酸蛋白激酶和正常细胞酪氨酸蛋白 激活剂。这些研究将使我们能够定义结构和 Pp60src的功能域，并允许我们将此信息与 其他可能是相关酪氨酸蛋白家族成员的蛋白质 激活剂。