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CLINICO-BIOLOGICAL CORRELATION IN LYMPHOMA AND LEUKEMIA

淋巴瘤和白血病的临床生物学相关性

基本信息

批准号：
3167319
负责人：
KHALIL SHEIBANI
金额：
$ 15.83万
依托单位：
CITY OF HOPE NATIONAL MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
1983
资助国家：
美国
起止时间：
1983-05-01 至 1988-03-31
项目状态：
已结题

项目摘要

The goal is to study human leukemias and non-Hodgkin's lymphomas using a multidisciplinary approach encompassing morphology, immunologic markers, cytogenetics, and flow microfluorometric analysis of DNA content, cell size, and cell kinetics in order to improve characterization and classification of these diseases. Cell suspensions and frozen tissues from more than 750 cases of non-Hodgkin's lymphomas, lymphocytic leukemias, and reactive lymphoproliferative conditions have been studied during the past year. We measured adenosine deaminase and purine nucleoside phosphorylase activities by using a linked enzyme spectrophotometric assay on peripheral blood leukemia cells from seven patients with chronic lymphocytic leukemia, three patients with prolymphocytic leukemia and one patient with prolymphocytoid transformation of chronic lymphocytic leukemia. Our studies suggest that purine nucleoside phosphorylase activity in leukemic cells may be useful in distinguishing prolymphocytic leukemia from chronic lymphocytic leumemia, and that it may be an enzymatic marker for the early detection of prolymphocytoid transformation of chronic lymphocytic leukemia. We have described the presence of monocytoid B lymphocytes (MBL) in 17 of 22 lymph nodes from 20 patients with Acquired Immune Deficiency Syndrome (AIDS) and AIDS-related lymphadenopathy. In all 17 samples, the MBL were found in lymph nodes showing florid reactive follicular hyperplasia. MBL were not detected, however, in lymph nodes showing involuted follicles or lymphocyte depletion. Disappearance of MBL apparently parallels the progressive involution of secondary follicles. The diminished T-helper status in AIDS may be relevant to the pathogenesis of follicular involution and indirectly to the disappearance of MBL. Peripheral T-cell lymphomas (PTCL) have been shown to be a morphologically heterogenous group of non-Hodgkin's lymphomas. We have studied PTCL occurring in a 13-year-old girl from whom we were able to evaluate histologic material from 11 biopsies obtained during her 8-month clincial course. These biopsies demonstrated morphologic progression from atypical immune reactions (AIR) and diffuse mixed cell lymphoma (DM) at the time of clinical diagnosis to pleomorphic large cell lymphoma (LCL) with erythrophagocytic tumor cells at the time of her death. We conclude that morphologic progression in PTCL is expressed, in sequential biopsies, by a progressive cellular pleomorphism with the appearance of large, often bizarre lymphoid cells, and the gradual diminuition and disappearance of the B-cell areas and epitheloid histiocytic areas which were present initially. (4)

目标是使用一种方法来研究人类白血病和非霍奇金淋巴瘤多学科方法，包括形态学、免疫学标记、细胞遗传学、DNA 含量、细胞的流式显微荧光分析尺寸和细胞动力学，以改善表征和这些疾病的分类。来自 750 多例的细胞悬液和冷冻组织非霍奇金淋巴瘤、淋巴细胞白血病和反应性白血病去年对淋巴增殖性疾病进行了研究。我们测量腺苷脱氨酶和嘌呤核苷磷酸化酶活性通过对外周血使用联酶分光光度测定法来自七名慢性淋巴细胞白血病患者的白血病细胞，其中三名幼淋巴细胞白血病患者和幼淋巴细胞样白血病患者 1 例慢性淋巴细胞白血病的转化。我们的研究表明白血病细胞中的嘌呤核苷磷酸化酶活性可能有助于区分幼淋巴细胞白血病和慢性淋巴细胞白血病，它可能是早期检测的酶标记慢性淋巴细胞白血病的幼淋巴细胞样转化。我们有描述了 22 个淋巴中的 17 个存在单核细胞样 B 淋巴细胞 (MBL) 来自 20 名获得性免疫缺陷综合症 (AIDS) 患者的节点艾滋病相关的淋巴结病。在所有 17 个样本中，均发现了 MBL 淋巴结显示华丽的反应性滤泡增生。 MBL 没有然而，在显示退化滤泡或淋巴细胞的淋巴结中检测到消耗。 MBL 的消失显然与进步主义平行次级卵泡退化。 T辅助细胞在艾滋病中的地位下降可能与卵泡复旧的发病机制有关并间接相关导致MBL的消失。外周 T 细胞淋巴瘤 (PTCL) 显示为非霍奇金病的形态异质组淋巴瘤。我们研究了一名 13 岁女孩发生的 PTCL 我们能够评估 11 个活检组织的组织学材料在她为期 8 个月的临床课程中。这些活检表明非典型免疫反应（AIR）和弥漫性免疫反应的形态学进展临床诊断为多形性混合细胞淋巴瘤 (DM) 大细胞淋巴瘤 (LCL) 时伴有吞噬红细胞肿瘤细胞她的死。我们得出的结论是 PTCL 的形态学进展是这样表达的：在连续活检中，通过渐进的细胞多形性出现大的、通常奇怪的淋巴细胞，并且逐渐 B 细胞区域和上皮样细胞减少和消失最初存在的组织细胞区域。 (4)