CLINICO-BIOLOGICAL CORRELATION IN LYMPHOMA AND LEUKEMIA

淋巴瘤和白血病的临床生物学相关性

• 批准号: 3167318
• 负责人: CARL D WINBERG
• 金额: $ 14.84万
• 依托单位: CITY OF HOPE NATIONAL MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-05-01 至 1988-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3167318
• 关键词: bone marrow exam; cell bank /registry; cell population study; computer graphics /printing; cytodiagnosis; diagnosis quality /standard; early diagnosis; electron microscopy; flow cytometry; fluorescence microscopy; fluorescent dye /probe; human subject; immune adherence reaction; immunoelectrophoresis; immunofluorescence technique; karyotype; leukemia; lymphatic tissue; lymphocyte; lymphoma; monoclonal antibody; neoplasm /cancer classification /staging; neoplasm /cancer diagnosis; neoplasm /cancer immunodiagnosis; neoplasm /cancer immunology; neoplastic growth; nucleic acid structure; surface antigens; tissue /cell culture

The goal is to study human leukemias and non-Hodgkin's lymphomas using a multidisciplinary approach encompassing morphology, immunologic markers, cytogenetics, and flow microfluorometric analysis of DNA content, cell size, and cell kinetics in order to improve characterization and classification of these diseases. Cell suspensions and frozen tissues from more than 750 cases of non-Hodgkin's lymphomas, lymphocytic leukemias, and reactive lymphoproliferative conditions have been studied during the past year. We measured adenosine deaminase and purine nucleoside phosphorylase activities by using a linked enzyme spectrophotometric assay on peripheral blood leukemia cells from seven patients with chronic lymphocytic leukemia, three patients with prolymphocytic leukemia and one patient with prolymphocytoid transformation of chronic lymphocytic leukemia. Our studies suggest that purine nucleoside phosphorylase activity in leukemic cells may be useful in distinguishing prolymphocytic leukemia from chronic lymphocytic leumemia, and that it may be an enzymatic marker for the early detection of prolymphocytoid transformation of chronic lymphocytic leukemia. We have described the presence of monocytoid B lymphocytes (MBL) in 17 of 22 lymph nodes from 20 patients with Acquired Immune Deficiency Syndrome (AIDS) and AIDS-related lymphadenopathy. In all 17 samples, the MBL were found in lymph nodes showing florid reactive follicular hyperplasia. MBL were not detected, however, in lymph nodes showing involuted follicles or lymphocyte depletion. Disappearance of MBL apparently parallels the progressive involution of secondary follicles. The diminished T-helper status in AIDS may be relevant to the pathogenesis of follicular involution and indirectly to the disappearance of MBL. Peripheral T-cell lymphomas (PTCL) have been shown to be a morphologically heterogenous group of non-Hodgkin's lymphomas. We have studied PTCL occurring in a 13-year-old girl from whom we were able to evaluate histologic material from 11 biopsies obtained during her 8-month clincial course. These biopsies demonstrated morphologic progression from atypical immune reactions (AIR) and diffuse mixed cell lymphoma (DM) at the time of clinical diagnosis to pleomorphic large cell lymphoma (LCL) with erythrophagocytic tumor cells at the time of her death. We conclude that morphologic progression in PTCL is expressed, in sequential biopsies, by a progressive cellular pleomorphism with the appearance of large, often bizarre lymphoid cells, and the gradual diminuition and disappearance of the B-cell areas and epitheloid histiocytic areas which were present initially. (4)

其目标是利用一种新的免疫学方法研究人类白血病和非霍奇金淋巴瘤。 多学科方法，包括形态学，免疫学标记， 细胞遗传学和DNA含量的流式显微荧光分析，细胞 尺寸和细胞动力学，以改善表征， 这些疾病的分类。 来自750多个病例的细胞悬液和冷冻组织 非霍奇金淋巴瘤、淋巴细胞白血病和反应性 在过去的一年中，已经研究了淋巴增生性病症。 我们 测定腺苷脱氨酶和嘌呤核苷磷酸化酶活性 用酶联分光光度法测定外周血 来自7名慢性淋巴细胞白血病患者的白血病细胞， 2例前淋巴细胞白血病和1例前淋巴细胞样白血病 慢性淋巴细胞白血病的转化。 我们的研究表明， 白血病细胞中嘌呤核苷磷酸化酶活性可用于 区分幼淋巴细胞白血病和慢性淋巴细胞白血病， 它可能是一种酶标记，用于早期检测 慢性淋巴细胞白血病的前淋巴细胞样转化。 我们有 描述了22例淋巴结中17例存在单核细胞样B淋巴细胞（MBL 20例获得性免疫缺陷综合征（AIDS）患者的淋巴结， 艾滋病相关淋巴结病。 在所有17个样本中，MBL被发现， 淋巴结表现出鲜艳的反应性滤泡增生。 MBL不是 然而，在显示退化滤泡或淋巴细胞的淋巴结中检测到 耗尽 MBL的消失显然与 次级卵泡退化。 艾滋病患者辅助性T细胞减少 可能与卵泡退化的发病机制有关， 与MBL的失踪有关 外周T细胞淋巴瘤（PTCL）已被 显示为非霍奇金淋巴瘤的形态异质性组 淋巴瘤 我们研究了发生在一个13岁女孩的PTCL， 我们能够评估11个活检组织的组织学材料， 在她8个月的临床课程中。 这些活组织检查显示 从非典型免疫反应（AIR）和弥漫性 混合细胞淋巴瘤（DM）在临床诊断时为多形性 大细胞淋巴瘤（LCL）伴吞噬红细胞肿瘤细胞， 她的死 我们的结论是PTCL的形态学进展， 在连续活检中，通过进行性细胞多形性， 出现大的，通常是奇异的淋巴细胞，以及逐渐的 B细胞区和上皮样细胞减少和消失 最初存在的组织细胞区域。 （四）