ANTHRAMYCIN--PHYSIOLOGY OF PRODUCTION

红霉素——生产的生理学

• 批准号: 3169510
• 负责人: LAURENCE H. HURLEY
• 金额: $ 13.7万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-05-01 至 1986-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3169510
• 关键词: Bloom syndrome; DNA; X ray crystallography; antineoplastic antibiotics; ataxia telangiectasia; chemical binding; chemical structure function; chromatin; conformation; congenital aplastic anemia; crystallization; density gradient ultracentrifugation; dinucleotide; drug adverse effect; drug design /synthesis /production; endonuclease; gel electrophoresis; genetic disorder; human tissue; molecular cloning; nuclear magnetic resonance spectroscopy; nucleosomes; pharmacogenetics; photolysis; plasmids; radiation sensitivity; radiotracer; simian virus 40; ultraviolet radiation; virus DNA; virus replication; xeroderma pigmentosum

OBJECTIVES: This research is a continuation of previous work carried out to elucidate the manner in which anthramycin and related drugs react with DNA and the biological consequences of these reactions. Our broad objectives during the next period are: 1) to precisely characterize t damage to DNA and chromatin caused by anthramycin, 2) to explore and characterize the biological consequences of this damage using human cell lines and replicative SV-40 DNA synthesis, and 3) to examine the genetics of production and resistance to anthramycin by the producing organism. APPROACH: DNA damage by anthramycin will be probed using 1H-NMR with synthetic polydeoxynucleotides, and at the chromatin level by biochemical techniques. Biological consequences (DNA repair parameters, double strand breaks, inhibition of replication) will be followed in human cell lines (normal, XP, AT and FA) and in a permissive SV-40 replication system. Plasmid and chromosomal DNA will be examined from the anthramycin producing organism.

目的：本研究是以往工作的延续

项目成果

Reaction of anthramycin with DNA. Biological consequences of DNA damage in normal and xeroderma pigmentosum cell.

蒽霉素与 DNA 的反应。

• 发表时间: 1982
• 期刊: The Journal of biological chemistry
• 作者: Petrusek,RL;Uhlenhopp,EL;Duteau,N;Hurley,LH
• 通讯作者: Hurley,LH

The reaction of anthramycin with DNA. Proton and carbon nuclear magnetic resonance studies on the structure of the anthramycin-DNA adduct.

蒽霉素与 DNA 的反应。

• 发表时间: 1984
• 期刊: The Journal of biological chemistry
• 作者: Graves,DE;Pattaroni,C;Krishnan,BS;Ostrander,JM;Hurley,LH;Krugh,TR
• 通讯作者: Krugh,TR