CHARACTERIZATION OF NON-HLA PLATELET ANTIGENS/ANTIBODIES

非 HLA 血小板抗原/抗体的表征

• 批准号: 3171187
• 负责人: Sherrill J. Slichter
• 金额: $ 7.55万
• 依托单位: BLOODWORKS
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-09-30 至 1987-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3171187
• 关键词: adult human (21+); affinity chromatography; antibody specificity; blood donor; computer assisted diagnosis; genetic markers; histocompatibility antigens; histocompatibility gene; histocompatibility typing; human population genetics; human subject; immunoglobulin G; immunohematology; infant human (0-1 year); leukocyte antigen typing; monoclonal antibody; platelet transfusion; plateletpheresis; purpura; radioimmunoassay; radiotracer; thrombocytopenia

The ultimate goal of this proposal is to identify the non-HLA platelet antigens that must be matched between donor and recipient to ensure platelet compatibility. This will be done by careful characterization of non-HLA platelet antibodies and antigens. Initially we will identify and categorize non-HLA platelet antisera. This will involve: 1) develop sensitive, reliable and comprehensive platelet antibody testing techniques; 2) evaluate platelet crossmatch tests to determine the transfusion relevant antibodies/angigens; 3) obtain antisera from appropriate sources (i.e., patients with post-transfusion purpura, mothers of infants with neonatal alloimmune thrombocytopenia, multiparous women and from planned platelet immunization programs); 4) test the sera with selected platelet panels to group the antisera into those detecting the same antigens; and 5) produce the relevant antisera in quantity using hybridoma technology. These well-characterized non-HLA platelet antisera will be used in family studies to detect the corresponding antigens. The same families will be tested for other genetic markers (HLA antigens, red cell antigens, red cell enzymes and plasma proteins) to determine the inheritance and genetic linkage and/or chromosomal assignment of non-HLA platelet antigens. Further analysis of non-HLA platelet antigens will be done by determining their expression on other body cells as well as their localization to specific glycoprotein fractions within the platelet membrane.

这一建议的最终目的是识别非人类白细胞抗原(Non-HLA)血小板 供体和受体之间必须匹配的抗原，以确保 血小板相容性。这将通过仔细描述 非人类白细胞抗原和血小板抗体。最初，我们将确定和 对非人类白细胞抗原抗血清进行分类。这将涉及：1)开发 灵敏、可靠、全面的血小板抗体检测 技术；2)评估血小板交叉匹配试验以确定 输注相关抗体/血管紧张素；3)获得抗血清 适当的来源(即，输血后紫癜患者、母亲 新生儿同种免疫性血小板减少症的婴儿、经产妇女和 从计划的血小板免疫计划中)；4)检测血清 选定的血小板面板将抗血清分组为那些检测 相同的抗原；5)大量生产相应的抗血清 杂交瘤技术。这些具有良好特征的非人类白细胞抗原抗血清 将用于家庭研究，以检测相应的抗原。这个 将对同一个家族进行其他遗传标记(人类白细胞抗原、红色 细胞抗原、红细胞酶和血浆蛋白)来确定 非人类白细胞抗原的遗传和遗传连锁和/或染色体分配 血小板抗原。对非人类白细胞抗原的进一步分析将是 通过确定它们在其他身体细胞上的表达以及它们的 对血小板内特定糖蛋白部分的定位 薄膜。