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INAPPROPRIATE H-2 (K/D) AND IE/C ANTIGENS ON TUMORS

肿瘤上不适当的 H-2 (K/D) 和 IE/C 抗原

基本信息

批准号：
3169721
负责人：
BENJAMIN BONAVIDA
金额：
$ 7.5万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
1982
资助国家：
美国
起止时间：
1982-05-01 至 1986-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3169721
关键词：
gene expression histocompatibility antigens host neoplasm interaction immunochemistry leukocyte activation /transformation monoclonal antibody neoplasm /cancer immunology neoplastic growth sarcoma tissue /cell culture

项目摘要

The objectives of our studies are to investigate the immunological functions and molecular nature of alien H-2 specificities that we have detected on primary spontaneous and transplantable reticulum cell sarcoma (RCS) of SJL/J (H-25) mice. RCS has been shown to express public H-2K/O antigens that are cryptic on normal SJL/J cells by cellular, serological, and immunochemical procedures. Furthermore, RCS express hybrid Ia antigens coded for by the Ia (Ae beta chain) and IE/C (E alpha chain) subregions, which are not normally expressed on host SJL/J (H-25) cells but are present on lymphocytes of other haplotypes. In this investigation, we plan to define the molecular nature of the crossreactive H-2 antigens using monospecific and monoclonal antibodies in order to determine if they are associated with the H-2K or D molecules. We will also define the biochemical nature of the hybrid IE/C antigen and its relationship to hybrid Ia of other H-2d and H-2k haplotypes. One-\and two-dimensional PAGE will be done and supplemented by peptide mappings. The role of alien antigens of RCS in the stimulation of syngeneic lymphocytes will be examined by the use of various recombinant strains of F1 hybrids as responder and stimulator cells. The nature of stimulating antigens will be investigated by examining the effect of monoclonal antibodies on the proliferative response in vitro. Furthermore, the role of passive transfer of antialien antibodies on the in vivo tumor growth will be analyzed. (AG)

我们研究的目的是调查免疫学我们所拥有的外来 H-2 特异性的功能和分子性质在原发性自发性和可移植性网状细胞肉瘤中检测到 SJL/J (H-25) 小鼠的 (RCS)。 RCS已被证明可以表达公共H-2K/O 通过细胞学、血清学、和免疫化学程序。此外，RCS 表达杂合 Ia 抗原由 Ia（Ae β 链）和 IE/C（E α 链）亚区编码，通常在宿主 SJL/J (H-25) 细胞上不表达，但存在对其他单倍型的淋巴细胞。在本次调查中，我们计划使用定义交叉反应 H-2 抗原的分子性质单特异性和单克隆抗体，以确定它们是否与 H-2K 或 D 分子相关。我们还将定义杂合 IE/C 抗原的生化性质及其与其他 H-2d 和 H-2k 单倍型的杂种 Ia。一维和二维 PAGE 将通过肽图谱进行并补充。外星人的角色 RCS 抗原在刺激同基因淋巴细胞时将通过使用 F1 杂种的各种重组菌株进行检查反应细胞和刺激细胞。刺激抗原的性质是通过检查单克隆抗体对体外增殖反应。此外，被动转移的作用将分析抗外来抗体对体内肿瘤生长的影响。 (AG)