CHARACTERIZATION OF NON-HLA PLATELET ANTIGENS/ANTIBODIES

非 HLA 血小板抗原/抗体的表征

• 批准号: 3171188
• 负责人: Sherrill J. Slichter
• 金额: $ 7.5万
• 依托单位: BLOODWORKS
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-09-30 至 1987-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3171188
• 关键词: adult human (21+); affinity chromatography; antibody specificity; blood donor; computer assisted diagnosis; genetic markers; histocompatibility antigens; histocompatibility gene; histocompatibility typing; human population genetics; human subject; immunoglobulin G; immunohematology; infant human (0-1 year); leukocyte antigen typing; monoclonal antibody; platelet transfusion; plateletpheresis; purpura; radioimmunoassay; radiotracer; thrombocytopenia

The ultimate goal of this proposal is to identify the non-HLA platelet antigens that must be matched between donor and recipient to ensure platelet compatibility. This will be done by careful characterization of non-HLA platelet antibodies and antigens. Initially we will identify and categorize non-HLA platelet antisera. This will involve: 1) develop sensitive, reliable and comprehensive platelet antibody testing techniques; 2) evaluate platelet crossmatch tests to determine the transfusion relevant antibodies/angigens; 3) obtain antisera from appropriate sources (i.e., patients with post-transfusion purpura, mothers of infants with neonatal alloimmune thrombocytopenia, multiparous women and from planned platelet immunization programs); 4) test the sera with selected platelet panels to group the antisera into those detecting the same antigens; and 5) produce the relevant antisera in quantity using hybridoma technology. These well-characterized non-HLA platelet antisera will be used in family studies to detect the corresponding antigens. The same families will be tested for other genetic markers (HLA antigens, red cell antigens, red cell enzymes and plasma proteins) to determine the inheritance and genetic linkage and/or chromosomal assignment of non-HLA platelet antigens. Further analysis of non-HLA platelet antigens will be done by determining their expression on other body cells as well as their localization to specific glycoprotein fractions within the platelet membrane.

本研究的最终目的是鉴定非HLA血小板 抗原必须在供体和受体之间匹配，以确保 血小板相容性 这将通过仔细描述 非HLA血小板抗体和抗原。 首先，我们将确定和 分类非HLA血小板抗血清。 这将涉及：1）开发 灵敏、可靠、全面的血小板抗体检测 技术; 2）评估血小板交叉配型试验，以确定 输血相关抗体/血管生成素; 3）从以下获得抗血清： 适当的来源（即，输血后紫癜患者，母亲 新生儿同种免疫性血小板减少症的婴儿， 来自计划的血小板免疫程序）; 4）用 选择血小板板，将抗血清分组为检测 相同的抗原;和5）使用 杂交瘤技术。 这些充分表征的非HLA血小板抗血清 将用于家族研究，以检测相应的抗原。 的 同样的家庭将测试其他遗传标记（HLA抗原，红 细胞抗原、红细胞酶和血浆蛋白），以确定 非HLA遗传和遗传连锁和/或染色体分配 血小板抗原 将进一步分析非HLA血小板抗原 通过确定它们在其他体细胞上的表达以及它们的 定位于血小板内的特定糖蛋白组分 膜的