FUNCTIONAL & PHENOTYPIC ANALYSIS OF T-CELLS IN FIV-FAIDS

一种类似人类免疫缺陷病毒(HIV)的猫慢病毒 在我们实验室从免疫缺陷家猫身上分离出来的 综合征，并被命名为猫免疫缺陷病毒(FIV)。标志性的 猫感染FIV的一个原因是感染者的易感性增加 动物对继发性感染的反应与已观察到的非常相似 用于人类感染艾滋病毒的药物。此外，还给出了实验结果 这表明受感染猫的T细胞免疫功能存在缺陷。对.的使用 FIV感染猫作为人类艾滋病的小动物模型应 因此被考虑，因为它更经济，更快， 人类艾滋病的模型比猫白血病病毒的模型更接近 或被其他动物慢病毒感染，灵长类动物模型除外。 本项目的目的是利用艾滋病的FIV模型来评估 T淋巴细胞在FIV-FAIDS免疫发病机制中的作用在这 研究，猫科试剂和功能分析需要评估猫科T 发展淋巴细胞网络。这些试剂和程序将 然后用于监测FIV各个阶段的T细胞功能 猫的感染。这项研究还将尝试解决以下问题 目前尚不清楚的FIV感染点：(1) 感染FIV的T细胞亚群(S)及T细胞作为 抗原提呈细胞。(2)如果T细胞亚群确实存在缺陷 那么这些缺陷在FIV免疫发病机制中的作用- 法艾德。该项目的总体意义在于确定 艾滋病在动物中发生的机制(S)和免疫学的发展 可用于预防艾滋病病毒感染的方法。这个 这些研究的结果应该有助于确定预防和治疗 将有助于抗击人类艾滋病毒感染的治疗战略。

A feline lentivirus resembling human immunodeificency virus (HIV) was siolated in our laboratory from domestic cats with immunodeficiency syndrome and was named feline immunodeiciency virus (FIV). The hallmark of FIV infection in cats is the increased susceptibility of the infected animals to secondary infections much similar to what has been observed for HIV infection of humans. In addition, there are experimental results suggesting a defect in T cell immunity in the infected cats. The use of FIV infection of cats as a small animal model for human AIDS should therefore be considered because it is far more economical, more rapid, and much closer model for human AIDS that those of feline leukemia virus or by other animal lentiviruses with the exception of the primate models. The purpose of this project is to use the FIV model of AIDs to evaluate the role of T lymphocytes in the immunopathogenesis of FIV-FAIDS. In this study, feline reagents and functional assays needed to evaluate feline T lymphocyte network will be developed. These reagents and procedures will then be used to monitor the T-cell functions during various stages of FIV infection in cats. This study will also attempt to address the following points of FIV infection which is still unknown: (1) The identity of the T-cell subset(s) that is/are infected by FIV and the role of T cells as antigen presenting cells. (2) If subsets of T-cells deficiencies do exist then the role of these deficiencies in the immunopathogensis of FIV- FAIDS. The overall significance of this project is to identify the mechanism(s) by which AIDS occurs in animals and to develop immunological approaches that could be used to prevent AIDS virus infection. The results from these studies should help identify prophylaptic and therapeutic strategies that will help combat HIV infection of humans.