REPLICON CONTROL IN NORMAL AND TRANSFORMING CELLS

细小病毒与其宿主细胞的相互作用正被用作探针 真核细胞的分化、DNA复制和衰老。 我们将 探索细小病毒早期和晚期启动子的特性， 他们之间的互动。 早期启动子产生基因产物， 正调控晚期启动子。 机制和顺序 将研究参与这种反式激活的。 细小病毒早期 将测试启动子对宿主细胞调节蛋白的依赖性 和适于克隆分离的这种蛋白质的基因的细胞系。 在成纤维细胞分化的终末阶段， 对细小病毒感染具有抵抗力，这些细胞将被检测 病毒早期启动子活性的限制。

The interaction of parvovirus with its host cell is being used as a probe of eukaryotic cell differentiation, DNA replication, and aging. We will explore the properties of parvovirus early and late promoters and the interaction between them. The early promoter produces a gene product that positively regulates the late promoter. The mechanisms and sequences involved in this trans-activation will be studied. The parvovirus early promoter will be tested for dependence on a host cell regulatory protein and cell lines suitable for cloning the gene for such a protein isolated. In the terminal stages of fibroblast differentiation, the senescing cells become resistant to parvovirus infection and these cells will be tested for restriction of virus early promoter activity.