正常细胞和转化细胞中的复制子控制-猫眼课题宝

正常细胞和转化细胞中的复制子控制

批准号：

3175315

负责人：

SOLON L RHODE

金额：

$15.29万

依托单位：

UNIVERSITY OF NEBRASKA MEDICAL CENTER

依托单位国家：

美国

项目类别：

财政年份：

1985

资助国家：

美国

项目状态：

已结题

起止时间：

1985-07-01 至 1988-06-30

来源：

https://reporter.nih.gov/project-details/3175315

关键词：

Parvoviridae aging cell differentiation cell free system eukaryote fibroblasts gene expression genetic library genetic manipulation genetic recombination genetic transcription molecular cloning mutant nucleic acid sequence plasmids tissue /cell culture transforming virus virus genetics virus replication

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英文摘要

The interaction of parvovirus with its host cell is being used as a probe of eukaryotic cell differentiation, DNA replication, and aging. We will explore the properties of parvovirus early and late promoters and the interaction between them. The early promoter produces a gene product that positively regulates the late promoter. The mechanisms and sequences involved in this trans-activation will be studied. The parvovirus early promoter will be tested for dependence on a host cell regulatory protein and cell lines suitable for cloning the gene for such a protein isolated. In the terminal stages of fibroblast differentiation, the senescing cells become resistant to parvovirus infection and these cells will be tested for restriction of virus early promoter activity.

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