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Liverpool 3View: a national hub for 3D-EM bioscience research

Liverpool 3View：国家 3D-EM 生物科学研究中心

基本信息

批准号：
BB/L014416/1
负责人：
Ian Prior
金额：
$ 26.5万
依托单位：
University of Liverpool
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2013
资助国家：
英国
起止时间：
2013 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FL014416%2F1
关键词：
Liverpool 3View national hub 3D

项目摘要

New 3View equipment is required for imaging the fine details of the insides of cells and tissues in 3D. In all of our projects we are using multidisciplinary approaches to address our questions however they are all united by the need for imaging at high resolution. The challenge with this is that the closer you look the harder it is to see the surrounding context. The solution is to take images of many serial sections through the sample and then digitally reconstruct them into a 3D volume that can be viewed from any angle. This helps with understanding the spatial relationships between cells within tissues, between subcellular compartments and for observing rare events. The 3View system makes this type of analysis routine, allowing rapid processing of samples. Our projects span a broad range of subjects from basic cell biology and physiology such how cells organise their communication systems when they are moving, how the recycling machinery of the cell works, changes to cells and tissues during ageing and the biogenesis and structural organisation of healthy tissues. Our work also extend through to translational projects, for example interactions between engineered stem cells and host tissue, development of novel cell scaffolds for treating age-related eye disorders, infection routes taken by parasites or bacteria in the gut and the development of novel nanoparticle tools for delivering cargoes to cells. The equipment will be housed in a leading UK electron microscopy unit and available to external scientists from the UK and Europe. 3View is a new technology that is only beginning to become established within the UK. As part of making Liverpool a national hub for this technology and to help drive innovative applications, we will set up a workshop programme that will act as a forum for experienced users and will enable new users to learn about sample processing, imaging and analysis.

需要新的 3View 设备对细胞和组织内部的精细细节进行 3D 成像。在我们所有的项目中，我们都使用多学科方法来解决我们的问题，但它们都因高分辨率成像的需求而结合在一起。这样做的挑战在于，你看得越近，就越难看清周围的环境。解决方案是拍摄样品中许多连续切片的图像，然后将它们数字化重建为可以从任何角度查看的 3D 体积。这有助于理解组织内细胞之间、亚细胞区室之间的空间关系以及观察罕见事件。 3View 系统使这种类型的分析成为常规，从而可以快速处理样品。我们的项目涵盖基础细胞生物学和生理学等广泛的主题，例如细胞在移动时如何组织通信系统、细胞的回收机制如何工作、衰老过程中细胞和组织的变化以及健康组织的生物发生和结构组织。我们的工作还延伸到转化项目，例如工程干细胞和宿主组织之间的相互作用、开发用于治疗与年龄相关的眼部疾病的新型细胞支架、肠道中寄生虫或细菌的感染途径以及开发用于将货物输送到细胞的新型纳米颗粒工具。该设备将安装在英国领先的电子显微镜装置中，可供英国和欧洲的外部科学家使用。 3View 是一项在英国才刚刚开始普及的新技术。作为使利物浦成为该技术的国家中心并帮助推动创新应用的一部分，我们将设立一个研讨会计划，该计划将作为经验丰富的用户的论坛，并使新用户能够了解样品处理、成像和分析。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Effect of Local Topography on Cell Division of Staphylococcus spp.

DOI：
10.3390/nano12040683
发表时间：
2022-02-18
期刊：
Nanomaterials (Basel, Switzerland)
影响因子：
0
作者：
Sorzabal-Bellido I;Barbieri L;Beckett AJ;Prior IA;Susarrey-Arce A;Tiggelaar RM;Fothergill J;Raval R;Diaz Fernandez YA
通讯作者：
Diaz Fernandez YA

DRP-1 functions independently of mitochondrial structural perturbations to facilitate BH3 mimetic-mediated apoptosis

DOI：
10.1038/s41420-019-0199-x
发表时间：
2019-07-17
期刊：
CELL DEATH DISCOVERY
影响因子：
7
作者：
Milani, Mateus;Beckett, Alison J.;Varadarajan, Shankar
通讯作者：
Varadarajan, Shankar

Functional analysis after rapid degradation of condensins and 3D-EM reveals chromatin volume is uncoupled from chromosome architecture in mitosis.

凝缩蛋白快速降解和 3D-EM 后的功能分析表明，染色质体积与有丝分裂中的染色体结构脱钩。

DOI：
10.1242/jcs.210187
发表时间：
2018-02-22
期刊：
Journal of cell science
影响因子：
4
作者：
Samejima K;Booth DG;Ogawa H;Paulson JR;Xie L;Watson CA;Platani M;Kanemaki MT;Earnshaw WC
通讯作者：
Earnshaw WC

Microtubule organization within mitotic spindles revealed by serial block face scanning electron microscopy and image analysis.

DOI：
10.1242/jcs.203877
发表时间：
2017-05-15
期刊：
Journal of cell science
影响因子：
4
作者：
Nixon FM;Honnor TR;Clarke NI;Starling GP;Beckett AJ;Johansen AM;Brettschneider JA;Prior IA;Royle SJ
通讯作者：
Royle SJ

3D-CLEM Reveals that a Major Portion of Mitotic Chromosomes Is Not Chromatin.

DOI：
10.1016/j.molcel.2016.10.009
发表时间：
2016-11-17
期刊：
MOLECULAR CELL
影响因子：
16
作者：
Booth, Daniel G.;Beckett, Alison J.;Molina, Oscar;Samejima, Itaru;Masumoto, Hiroshi;Kouprina, Natalay;Larionov, Vladimir;Prior, Ian A.;Earnshaw, William C.
通讯作者：
Earnshaw, William C.