ONTOGENETIC DEVELOPMENT OF LYMPHOCYTES

淋巴细胞的个体发育

• 批准号: 3176443
• 负责人: ULRICH G HAMMERLING
• 金额: $ 21.99万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-04-01 至 1987-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3176443
• 关键词: B lymphocyte; alleles; cell differentiation; fluorescence microscopy; histocompatibility antigens; hybridomas; immunofluorescence technique; immunogenetics; immunoglobulin A; immunoglobulin M; lymphoma; monoclonal antibody; secretory immune system; surface antigens; ultracentrifugation

The Ly-6 group of murine lymphocyte antigens comprises at least five antigens. They are grouped into a multigene cluster un the basis of the close linkage of their controlling genes. To determine the exact number of these genes and to gain insight into their structure and organization are the major aims of this proposal. Ongoing work focuses on the proteins that carry the Ly-6 antigen determinants. Current results indicate that two of the five Ly-6 antigens, namely Ly 6A.2 and Ly 6E.1 constitute alleles of one and the same antigen. This conclusion is based on identity in molecular weight, electrophoretic mobility, subunit structure, and sharing of nonpolymorphic determinants that are detected by a rat monoclonal anti-Ly6 antibody. A previous report by another group that Ly 6A is a single chain protein of molecular weight 33000 may have to be revised. Using affinity chromatography, we have succeeded in purifying Ly-6 E in preparative amounts adequate for amino acid sequencing. Once sufficient sequence information has been obtained, we will construct oligo-nucleotide probes with which we hope to isolate the Ly 6E gene. The question whether Ly 6A and Ly 6E are allelic can best be answered by sequencing the corresponding genes obtained from representative strains of mice, B6 versus BALB/c. However, if sufficient quantities of Ly 6A can be obtained, sequencing of a N-terminal stretch of the Ly 6A protein may provide a satisfactory answer. (LB)

Ly-6组小鼠淋巴细胞抗原至少由5个 抗原。它们被分成一个多基因簇，其基础是 它们的控制基因紧密连锁。确定的确切数量 为了深入了解这些基因的结构和组织 这项提议的主要目的是。正在进行的工作主要集中在 携带Ly-6抗原决定簇。目前的结果表明，其中两个 5个Ly-6抗原，即Ly 6A.2和Ly 6E.1构成了 一个相同的抗原。 这一结论是基于分子量、电泳法的一致性 非多态决定因素的流动性、亚基结构和共享 是由鼠抗Ly6单抗检测的。之前的一份报告 另一类认为Ly 6A是一种分子量的单链蛋白 33000可能需要修改。 用亲和层析法成功地纯化了Ly-6E。 足以进行氨基酸测序的制备量。一旦足够 已获得序列信息，我们将构建寡核苷酸 我们希望用来分离Ly 6E基因的探针。问题是是否 Ly 6A和Ly 6E是等位基因，最好的答案是测序 从代表小鼠品系获得的相应基因，B6与 Balb/c。然而，如果可以获得足够数量的Ly 6A， 对Ly 6A蛋白的N-末端延伸进行测序可以提供 令人满意的答案。(磅)