TUMOR-ASSOCIATED DNA MOVEMENT

肿瘤相关 DNA 运动

• 批准号: 3173935
• 负责人: ERIK SELSING
• 金额: $ 9.87万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-12-01 至 1986-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3173935
• 关键词: DNA; chromosome translocation; genetic library; genetic recombination; genetic transcription; human tissue; immunoglobulin genes; karyotype; lymphocytic leukemia; molecular oncology; neoplasm /cancer genetics; nucleic acid sequence

Increasing evidence suggests that oncogenesis may require specific genetic events, some of which may involve chromosomal translocations. We have found that, in many murine B-cell tumors, a segment of DNA is frequently translocated (tumor-associated recombining DNA or tar DNA). In two B-cell lines, tar DNA has recombined downstream from potentially active immunoglobulin gene promoters. Recombinations involving tar DNA are not related to c-myc gene translocations also seen in many B-cell tumors. Tar DNA recombinations are found much more frequently in lambda-producing B-cell lines than in kappa-producing tumors. It may be that tar recombination is a developmentally regulated event during B-cell maturation. We will investigate tar DNA recombination to elucidate the molecular mechanisms underlying chromosomal recombinations in B-cell tumors and to determine if these recombinations are important for tumorigenesis. DNA movement may play a significant role in the transformation process as well as in normal development and evolution. (D)

越来越多的证据表明，肿瘤的发生可能需要特定的遗传因素 事件，其中一些可能涉及染色体易位。 我们有 发现，在许多小鼠B细胞肿瘤中，一段DNA经常被 易位（肿瘤相关重组DNA或焦油DNA）。 在两个B细胞 焦油DNA已经从潜在的活性基因下游重组， 免疫球蛋白基因启动子。 涉及焦油DNA的重组则不然 与许多B细胞肿瘤中也出现的c-myc基因易位相关。 焦油 DNA重组在产生DNA的细胞中更常见。 B细胞系比产生kappa的肿瘤。 可能是焦油 重组是B细胞发育过程中的一个发育调节事件， 成熟 我们将研究焦油DNA重组，以阐明 B细胞肿瘤中染色体重组的分子机制 并确定这些重组对肿瘤发生是否重要。 DNA运动可能在转化过程中发挥重要作用， 以及正常的发育和进化。 （丁）