ONTOGENETIC DEVELOPMENT OF LYMPHOCYTES

鼠淋巴细胞抗原的Ly-6组包含至少五个 抗原 它们被聚成一个多基因簇， 它们的控制基因紧密相连。 为了确定 了解这些基因的结构和组织， 这一提议的主要目的。 正在进行的工作集中在蛋白质， 携带Ly-6抗原决定簇。 目前的研究结果表明， 5种Ly-6抗原，即Ly 6A.2和Ly 6E.1构成了 同一种抗原 这一结论是基于分子量，电语音， 流动性，提交结构，和共享的非多态性决定因素， 通过大鼠单克隆抗Ly 6抗体检测。 此前报道 另一组Ly 6A是分子量为1000的单链蛋白质， 33000可能需要修改。 利用亲和层析法，我们成功地从大肠杆菌中纯化了Ly-6 E。 制备量足以进行氨基酸测序。 一旦足够 序列信息已经获得，我们将构建寡核苷酸 我们希望用它来分离Ly 6 E基因。 这个问题 Ly 6A和Ly 6 E是等位基因，最好通过对Ly 6A和Ly 6 E基因进行测序来回答。 从小鼠的代表性品系获得的相应基因，B6与 BALB/c。 然而，如果可以获得足够量的Ly 6A， Ly 6A蛋白的N-末端延伸的测序可以提供 满意的答案。 （LB）

The Ly-6 group of murine lymphocyte antigens comprises at least five antigens. They are grouped into a multigene cluster un the basis of the close linkage of their controlling genes. To determine the exact number of these genes and to gain insight into their structure and organization are the major aims of this proposal. Ongoing work focuses on the proteins that carry the Ly-6 antigen determinants. Current results indicate that two of the five Ly-6 antigens, namely Ly 6A.2 and Ly 6E.1 constitute alleles of one and the same antigen. This conclusion is based on identity in molecular weight, electrophonetic mobility, submit structure, and sharing of nonpolymorphic determinants that are detected by a rat monoclonal anti-Ly6 antibody. A previous report by another group that Ly 6A is a single chain protein of molecular weight 33000 may have to be revised. Using affinity chromatography, we have succeeded in purifying Ly-6 E in preparative amounts adequate for amino acid sequencing. Once sufficient sequence information has been obtained, we will construct oligo-nucleotide probes with which we hope to isolate the Ly 6E gene. The question whether Ly 6A and Ly 6E are allelic can best be answered by sequencing the corresponding genes obtained from representative strains of mice, B6 versus BALB/c. However, if sufficient quantities of Ly 6A can be obtained, sequencing of a N-terminal stretch of the Ly 6A protein may provide a satisfactory answer. (LB)