DIETARY FIBER AND FAT IN BILE ACID EXCRETION

胆汁酸排泄中的膳食纤维和脂肪

• 批准号: 3181150
• 负责人: DANIEL D GALLAHER
• 金额: $ 6.84万
• 依托单位: NORTH DAKOTA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-01 至 1988-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3181150
• 关键词: Leguminoseae; cellulose; dietary fiber; dietary lipid; feces analysis; grain; high performance liquid chromatography; lignin; nutrition related neoplasm /cancer; nutrition related tag

Colon cancer is one of the most common forms of cancer. Epidemiological evidence indicates that its genesis is environmental in nature and that diet may greatly influence its incidence. Dietary fiber has been implicated as protective whereas dietary fat may promote colon cancer. The effect of these two dietary components may be mediated through a common point - fecal bile acids. Evidence exists that fecal bile acids are tumor promoting agents. The proposed project is designed to investigate the effect of dietary fiber, derived from cereals and legumes, as well as fat on fecal bile acids (concentration and total excretion), and on the bile acid pool size. Four feeding trials will be conducted. In trials 1-3, rats will be fed a fiber-free diet or diets containing a fiber rich fraction. In trial 1 the fiber sources will be the brans of hard red spring, durum, and soft white wheat. In trial 2 the brans of oats, rye, barley and corn will be fed. In trial 3 fiber-rich fractions from pinto, navy, and kidney beans will be fed. In trial 4 the purified fibers cellulose and lignin as well as a purified soluble dietary fiber fraction and a purified insoluble dietary fiber fraction will be fed. In all 4 trials, each diet described will be fed at a low and high (5 vs. 20%) level of fat. After 5 weeks of feeding, fecal samples will be collected for 5 days and the pooled sample assayed for bile acids by high performance liquid chromatography after extraction with acidified ethanol and partial purification with C18 Sep-Paks. The animals will then be killed and the stomach, liver, small intestine, cecum, and colon taken, the bile acids extracted and assayed for bile acids to determine pool size. In addition, colonic 1 alpha-dehydroxylase activity will be determined using a radioassay. Results will be analyzed by two-way analysis of variance, with multiple comparisons to be made using the Bonferroni t-test.

结肠癌是最常见的癌症之一。 流行病学 有证据表明，其成因是环境的性质， 饮食对发病率有很大影响。 膳食纤维一直是 而膳食脂肪可能促进结肠癌。 的 这两种饮食成分的作用可能通过共同的 粪便胆汁酸。 有证据表明，粪便胆汁酸是肿瘤 促进剂。 拟议项目旨在调查 膳食纤维的作用，来自谷物和豆类，以及脂肪 粪便胆汁酸（浓度和总排泄量）和胆汁 酸池大小。 将进行四次喂食试验。 在试验1-3中， 大鼠将被喂食无纤维饮食或含有富含纤维的饮食 分数 在试验1中，纤维来源将是硬红的麸皮 春小麦、硬粒小麦和软白色小麦。 在试验2中，燕麦，黑麦， 大麦和玉米将被喂养。 在试验3中，来自平托的富含纤维的级分， 海军和芸豆将被喂养。 在试验4中， 纤维素和木质素以及纯化的可溶性膳食纤维部分 并且将供给纯化的不溶性膳食纤维级分。 在所有4 试验中，描述的每种饮食将以低和高（5%对20%）水平喂养 脂肪。 喂养5周后，将收集粪便样本5 天，并通过高效液相色谱法测定合并样品的胆汁酸 用酸化乙醇和部分提取后进行液相色谱分析 用C18 Sep-Paks纯化。 动物将被杀死， 胃、肝、小肠、盲肠和结肠，胆汁酸 提取并测定胆汁酸以确定合并液大小。 此外，本发明还提供了一种方法， 结肠1 α-脱羟酶活性将使用 放射性分析 结果将通过双向方差分析进行分析， 使用Bonferroni t检验进行多重比较。