深入研究纳米纤维材料对于细胞的增殖与迁移的作用机制对于了解材料与细胞间的相互作用具有重要意义。项目组前期工作显示：改性聚己内酯基纳米纤维支架的拓扑结构与理化特性可显著影响角质形成细胞(keratinocytes, Kc)的生物学行为，此外，支架可激活Kc细胞Integrinβ1并促进细胞FAK(Tyr-397)磷酸化水平上调，但是纳米支架是否通过Integrinβ1激活FAK信号通路从而影响细胞的生物学行为还有待证实。因此，本项目在前期研究基础上，拟进一步探究支架的拓扑结构（纤维直径、孔径与取向性）与表面特性对于Kc细胞粘附、增殖与迁移的调控作用，阐明支架介导的integrinβ1与FAK分子在促进创面愈合中发挥作用的具体分子机制，本项目的开展有助于进一步阐明支架-细胞之间的相互作用分子机制。

An insightful understanding of the mechanism involving the effects of nanofibrous matrix on the adhesion, proliferation, and migration of(keratinocytes, Kc) is important for exploring the scaffold/cell interaction, our previous study showed that LBL modified electrospun polycaprolactone (PCL)-based nanofibrous matrices with different topographical feature and physicochemical characteristics could significantly affect the biological behavior of Kc attached, besides, the nanofibers could significantly activated integrinβ1 and promoted the expression of FAK(focal adhesion kinase) (Tyr 397), however, the underlying regulatory mechanism and biological effect of Kc cells remained unclear. Based on these findings, we plan to further confirm the effect of nanofibrous scaffold on the wound healing via in vivo model; and explore the regulatory effect of nanofibrous matrix with various topographical features (fiber diameter, pore size and fiber organization) and surface chemistry on the adhesion, proliferation, and migration of Kc cells, furthermore, we aimed to determine the regulatory involvement of integrin β1 and FAK in promoting the wound healing on nanofibrous matrices, this project wound be helpful to further elucidate the regulatory mechanisms of scaffold/cell interaction.