RADIOPROTECTANT COPPER COMPLEX, CU(11)(3,5-DIPS)2

辐射防护铜络合物，CU(11)(3,5-DIPS)2

• 批准号: 3180235
• 负责人: John Robert Joseph Sorenson
• 金额: $ 9.92万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-30 至 1988-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3180235
• 关键词: antibody specificity; chemical hypersensitivity; chemical synthesis; copper; gamma radiation; ionizing radiation; metal complex; radiation immunosuppression; radiation recovery; radiation sensitivity; radiopharmacology; radioprotective agents; tissue /cell culture

Long term objectives are to evaluate Bis 3,5-diisopropylsaliclatocopper (II) Cu(II)(3,5-dips)2 treatment regimens for radioprotection of cells grown in culture and mice irradiated with clinically relevant doses of X- and gamma-radiation (40-170 rads/minute to deliver 800 to 1000 rads), and evaluate effects of radiation and treatment (0, 60, 80, or 100 mg/kg) on essential metalloelement metabolism and immunocompetency of mice. Specific aims of this proposal are to synthesize sufficient quantities of Cu(II) (3,5-dips)2 to enable studies of its radioprotectant activity. Radioprotectant activity will be determined by measuring survival of irradiated cultured cells and whole-body irradiated mice. Survival and treatment regimen will be correlated for irradiated cells grown in culture. Altered essential metalloelement metabolism and immunocompetency will be determined for irradiated and treated mice. Survival, alteration of plasma, liver, spleen, intestine, brain, and bone marrow copper content as well as immunocompetency will be correlated for irradiated and treated mice. Routine synthetic procedures will be used to synthesize Cu(II)(3,5-dips)2. Atomic absorption spectrophotometric methods will be used to determine tissue copper content. Colony formation, as measured with Puck-Marcus single cell techniques, will be used to determine irradiated-cell survival. Survival will also be used to measure the in vivo radioprotectant activity. Immunocompetency will be determined by evaluating the abilities of different lymphocyte subclasses to repopulate hosts with determinations of spleen cell markers, mitogen responsiveness, and bone marrow colony forming units. Antibody responsiveness will be measured using the Jerne plaque-forming assay and determinations of cell-mediated immunity will be measured using oxazolone hypersensitivity and mixed lymphocyte reactivity. Appropriate statistical programs will be used to evaluate significance of differences found in these studies.

长期目标是评价双3，5-二异丙基水杨酸铜 (II)Cu（II）（3，5-dips）2处理方案用于细胞的辐射防护 在培养物中生长，并用临床相关剂量的X-射线照射小鼠， 和伽马辐射（40-170拉德/分钟，以提供800至1000拉德），以及 评价辐射和处理（0、60、80或100 mg/kg）对 必需金属元素代谢和免疫功能。 该提案的具体目标是合成足够量的 Cu（II）（3，5-dips）2，以研究其辐射防护活性。 放射防护剂活性将通过测量 辐照培养细胞和全身辐照小鼠。 生存和 治疗方案将与生长在 文化 改变的必需金属元素代谢和免疫能力 将对辐照和处理的小鼠进行测定。 生存、改变 血浆、肝、脾、肠、脑和骨髓铜含量 以及免疫能力将与辐射和治疗相关 小鼠 常规合成程序将用于合成Cu（II）（3，5-dips）2。 原子吸收分光光度法将用于测定 组织铜含量 菌落形成，用Puck-Marcus测量 单细胞技术，将用于确定辐照细胞 生存 还将使用存活率来测量体内的 辐射防护活性。 免疫能力将通过以下方式确定： 评估不同淋巴细胞亚类的再增殖能力 测定宿主的脾细胞标志物、有丝分裂原反应性， 和骨髓集落形成单位。 抗体反应性将是 使用Jerne空斑形成测定法测量， 将使用恶唑酮超敏反应来测量细胞介导的免疫 和混合淋巴细胞反应性。 适当的统计方案将 用于评价这些研究中发现的差异的显著性。

项目成果

Combination therapy with CuDIPS, Tween 80 and cyclophosphamide: effects on survival and tumor incidence in SJL/J mice.

CuDIPS、Tween 80 和环磷酰胺联合治疗：对 SJL/J 小鼠生存和肿瘤发生率的影响。

• 发表时间: 1990
• 期刊: In vivo (Athens, Greece)
• 作者: CrispensJr,CG;Sorenson,JR
• 通讯作者: Sorenson,JR

Pharmacokinetic distribution of 67Cu(II)2[3,5-diisopropyl(carboxy-14C)salicylate]4 among murine tissues.

67Cu(II)2[3,5-二异丙基(羧基-14C)水杨酸酯]4 在小鼠组织中的药代动力学分布。

• DOI: 10.2174/0929867043363992
• 发表时间: 2004
• 期刊: Current medicinal chemistry
• 影响因子: 4.1
• 作者: Blincoe,Clifton;Chidambaram,Mani;Salari,Hamid;Bond,KyleB;Evans,DorisT;Gray-Kaufman,RhondaA;Griffey,HalBrent;Tranby,SandraGilbreth;Garner-Johnson,Buffy;Lee,Tina;Sorenson,JohnRJ
• 通讯作者: Sorenson,JohnRJ

Copper complexes stimulate hemopoiesis and lymphopoiesis.

铜络合物刺激造血和淋巴细胞生成。

• DOI: 10.1007/978-1-4613-0537-8_18
• 发表时间: 1989
• 期刊: Advances in experimental medicine and biology
• 作者: Soderberg,LS;Barnett,JB;Sorenson,JR
• 通讯作者: Sorenson,JR

Treatment of reticulum cell sarcoma in SJL/J mice with Tween 80.

使用 Tween 80 治疗 SJL/J 小鼠网状细胞肉瘤。

• 发表时间: 1988
• 期刊: Anticancer research
• 影响因子: 2
• 作者: CrispensJr,CG;Sorenson,JR
• 通讯作者: Sorenson,JR

Radiation recovery agents: Cu(II), Mn(II), Zn(II), or Fe(III) 3,5-diisopropylsalicylate complexes facilitate recovery from ionizing radiation induced radical mediated tissue damage.

辐射恢复剂：Cu(II)、Mn(II)、Zn(II) 或 Fe(III) 3,5-二异丙基水杨酸盐​​复合物有助于从电离辐射引起的自由基介导的组织损伤中恢复。

• DOI: 10.1007/978-1-4684-5730-8_10
• 发表时间: 1990
• 期刊: Advances in experimental medicine and biology
• 作者: Sorenson,JJ;Soderberg,LS;Baker,ML;Barnett,JB;Chang,LW;Salari,H;Willingham,WM
• 通讯作者: Willingham,WM