HEAT-INDUCED RADIOSENSITIZATION
HEAT-INDUCED RADIOSENSITIZATION
批准号:
3188173
负责人:
RAYMOND L WARTERS
金额:
$14.06万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1987
资助国家:
美国
项目状态:
已结题
起止时间:
1987-09-30 至 1993-05-31
中文摘要
提案的总体目标是确定……的效果
英文摘要
The overall objective of the proposal is to determine the effect(s) of
radiosensitizing hyperthermic treatments on cell nuclear structure and
function. In particular we will determine the effects(s) of : 1) acute
heating (AH; 43 to 45 degrees); 2) step-down-heating (SDH; AH followed by
chronic exposure to 41 degrees); 3) chronic heating at 41 degrees (tolerance
development) before x-irradiation; and 4) chronic heating at 41 degrees
subsequent to x-irradiation on the induction and/or repair of radiation-
induced DNA double strand breaks (dsb), apurinic (AP) sites, DNA protein
crosslinks (DPC) and chromosome aberration induction in CHO cells grown in
monolayer. The hypothesis we will test is that a hyperthermia-induced
increase in the induction frequency, and/or inhibition of repair, of one or
more of these radiation-induced DNA lesions correlates with
radiosensitization at the cellular level. To date, this hypothesis has not
proven generally true in the case of DNA single strand breaks (ssb).
The induction frequency and repairability (rate and extent) of radiation-
induced lesions (ssb abd dsb) in specific DNA sequences will be measure in
control and heated cells. Bulk DNA will be isolated by alkaline/neutral
filter elution or alkaline sucrose gradient sedimentation from irradiated
cells, and cells during the repair of DNA lesions. Bulk DNA collected onto
nitrocellulose filters will be probed by filter hybridization against
nucleic acid probes for transcriptionally active and/or inert DNA sequences.
The possible role of heat-induced alterations of nuclear structure and/or
composition in the inhibition of DNA damage removal will also be assessed.
The kinetics of increase, and removal, of polypeptides in nucleosomal and
nonnculesomal chromatin DNA and in cell nuclear matrices will be determined
by exogenous nuclease digestion of DNA, centrifugal isolation of nuclear
components and polypeptide quantitation and characterization of
polyacrylamide gel electrophoresis. The extent to which hyperthermic
exposure alters the number and/or conformation of nuclear matrix DNA binding
sites will be determined by assessing the DNA "domain" size and frequency in
control and heated cells.
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Persistence of radiation-induced double-strand breaks in the DNA of heated CHO cells.
加热的 CHO 细胞 DNA 中辐射诱导的双链断裂持续存在。
DOI:
10.1080/09553009314551911
发表时间:
1993
期刊:
International journal of radiation biology
影响因子:
2.6
作者:
[Warters,RL]
通讯作者:
Warters,RL
DOI:
10.1002/jcp.1041540202
发表时间:
1993
期刊:
Journal of cellular physiology
影响因子:
5.6
作者:
[Chu,GL, Ross,G, Wong,RS, Warters,R, Dewey,WC]
通讯作者:
Dewey,WC
Repair of DNA strand breaks at hyperthermic temperatures in Chinese hamster ovary cells.
中国仓鼠卵巢细胞高温下 DNA 链断裂的修复。
DOI:
10.1080/09553009214550601
发表时间:
1992
期刊:
International journal of radiation biology
影响因子:
2.6
作者:
[Warters,RL, Axtell,J]
通讯作者:
Axtell,J
Tissue selenium levels in selenium-supplemented rats and their relevance in mammary cancer protection.
补硒大鼠的组织硒水平及其与乳腺癌保护的相关性。
DOI:
10.1093/carcin/10.5.921
发表时间:
1989
期刊:
Carcinogenesis
影响因子:
4.7
作者:
[Ip,C, Hayes,C]
通讯作者:
Hayes,C
Topoisomerase II activity in a DNA double-strand break repair deficient Chinese hamster ovary cell line.
DNA 双链断裂修复缺陷的中国仓鼠卵巢细胞系中的拓扑异构酶 II 活性。
DOI:
10.1016/0921-8777(91)90008-d
发表时间:
1991
期刊:
Mutation research
影响因子:
--
作者:
[Warters,RL, Lyons,BW, Li,TM, Chen,DJ]
通讯作者:
Chen,DJ
共 10 条
CORE--IRRADIATION FACILITY
-
批准号:6269228
-
项目类别:
-
资助金额:$7.27万
-
财政年份:1997
-
负责人:RAYMOND L WARTERS
-
依托单位:
CORE--IRRADIATION FACILITY
-
批准号:6236799
-
项目类别:
-
资助金额:$9.45万
-
财政年份:1997
-
负责人:RAYMOND L WARTERS
-
依托单位:
HEAT-INDUCED RADIOSENSITIZATION
-
批准号:3188172
-
项目类别:
-
资助金额:$9.24万
-
财政年份:1987
-
负责人:RAYMOND L WARTERS
-
依托单位:
HEAT-INDUCED RADIOSENSITIZATION
-
批准号:3188170
-
项目类别:
-
资助金额:$5.84万
-
财政年份:1987
-
负责人:RAYMOND L WARTERS
-
依托单位:
HEAT-INDUCED RADIOSENSITIZATION
-
批准号:3188171
-
项目类别:
-
资助金额:$5.87万
-
财政年份:1987
-
负责人:RAYMOND L WARTERS
-
依托单位:
HEAT-INDUCED RADIOSENSITIZATION
-
批准号:3188168
-
项目类别:
-
资助金额:$9.25万
-
财政年份:1987
-
负责人:RAYMOND L WARTERS
-
依托单位:
CHROMATIN REPLICATION AND HEAT-INDUCED CELL DEATH
-
批准号:3167111
-
项目类别:
-
资助金额:$16.49万
-
财政年份:1983
-
负责人:RAYMOND L WARTERS
-
依托单位:
CHROMATIN REPLICATION AND HEAT-INDUCED CELL DEATH
-
批准号:3167102
-
项目类别:
-
资助金额:$14.03万
-
财政年份:1983
-
负责人:RAYMOND L WARTERS
-
依托单位:
CHROMATIN REPLICATION AND HEAT-INDUCED CELL DEATH
-
批准号:3167112
-
项目类别:
-
资助金额:$17.16万
-
财政年份:1983
-
负责人:RAYMOND L WARTERS
-
依托单位:
CHROMATIN REPLICATION AND HEAT-INDUCED CELL DEATH
-
批准号:3167109
-
项目类别:
-
资助金额:$17.35万
-
财政年份:1983
-
负责人:RAYMOND L WARTERS
-
依托单位:
CHROMATIN REPLICATION AND HEAT-INDUCED CELL DEATH
-
批准号:3167108
-
项目类别:
-
资助金额:$14.44万
-
财政年份:1983
-
负责人:RAYMOND L WARTERS
-
依托单位:
CHROMATIN REPLICATION AND HEAT-INDUCED CELL DEATH
-
批准号:3167110
-
项目类别:
-
资助金额:$16.05万
-
财政年份:1983
-
负责人:RAYMOND L WARTERS
-
依托单位:
CHROMATIN REPLICATION AND HEAT-INDUCED CELL DEATH
-
批准号:3167106
-
项目类别:
-
资助金额:$12.73万
-
财政年份:1983
-
负责人:RAYMOND L WARTERS
-
依托单位:
CHROMATIN REPLICATION AND HEAT-INDUCED CELL DEATH
-
批准号:3167107
-
项目类别:
-
资助金额:$13.35万
-
财政年份:1983
-
负责人:RAYMOND L WARTERS
-
依托单位:
MECHANISM OF RADIOSENSITIATION BY STEP DOWN HEATING
-
批准号:4691247
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:RAYMOND L WARTERS
-
依托单位:
CORE--IRRADIATION FACILITY
-
批准号:6102275
-
项目类别:
-
资助金额:$8.47万
-
财政年份:--
-
负责人:RAYMOND L WARTERS
-
依托单位:
CORE--IRRADIATION FACILITY
-
批准号:3730645
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
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负责人:RAYMOND L WARTERS
-
依托单位:
CORE--IRRADIATION FACILITY
-
批准号:5207308
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
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负责人:RAYMOND L WARTERS
-
依托单位:--
海外基金