MASS SPECTROMETRY OF URINARY NUCLEOSIDES

尿核苷的质谱分析

• 批准号: 3184962
• 负责人: KARL H SCHRAM
• 金额: $ 11.7万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-30 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3184962
• 关键词: breast neoplasm /cancer diagnosis; colon neoplasms; early diagnosis; human therapy evaluation; human tissue; leukemia; mass spectrometry; neoplasm /cancer diagnosis; noninvasive diagnosis; nucleosides; urinalysis

Literature references attest to the potential utility of nucleoside levels in the urine of cancer patients as "biological markers" for the diagnosis and monitoring of cancer. However, current methods of analysis are prone to interferences and lack the sensitivity necessary to quantate "rare" nucleosides. GCMS methods will, therefore, be used to quantate selected nucleosides in the urine of cancer patients by the stable isotope dilution and metastable ion monitoring techniques. In addition, mass spectrometry will be used to determine the structures of unknown nucleosides in cancer patient urine which may provide a better understanding of the biochemistry of cancer cells. Urine of cancer patients will be provided by my clinical collaborators and will include samples from persons with breast cancer, cancer of the colon and leukemia. Urine samples will be obtained upon diagnosis, during treatment and following therapy. Results will then be compared to determine the utility of urinary nucleosides as "biological markers" of cancer. Samples will be prepared and analyzed using methods developed in this laboratory. The methods have been tested using urine from normal persons and are now ready to be applied to clinical studies. The research described in this proposal has the potential of providing 1) a simple, noninvasive, rapid method for the diagnosis of cancer, 2) clinicians with a noninvasive and safe method for following the patients response to therapy, and 3) an indication of relapse by the patient.

文献资料证明了核苷水平的潜在效用

项目成果

Isolation and identification of urinary nucleosides. Applications of high-performance liquid chromatographic methods to the synthesis of 5'-deoxyxanthosine and the simultaneous determination of 5,6-dihydrouridine and pseudouridine.

尿核苷的分离和鉴定。

• DOI: 10.1016/s0021-9673(01)89352-3
• 发表时间: 1990
• 期刊: Journal of chromatography
• 作者: Nakano,K;Yasaka,T;Schram,KH;Reimer,ML;McClure,TD;Nakao,T;Yamamoto,H
• 通讯作者: Yamamoto,H

Differentiation of isomeric 2'-, 3'- and 5'-deoxynucleosides by electron ionization and chemical ionization-linked scanning mass spectrometry.

通过电子电离和化学电离联扫描质谱法区分异构体 2-、3- 和 5- 脱氧核苷。

• DOI: 10.1002/bms.1200180804
• 发表时间: 1989
• 期刊: Biomedical & environmental mass spectrometry
• 作者: Reimer,ML;McClure,TD;Schram,KH
• 通讯作者: Schram,KH